基于唾液样本的 PCR 对新生儿巨细胞病毒感染的诊断试验准确性：系统评价和荟萃分析方案。
Diagnostic test accuracy of PCR by saliva specimen for cytomegalovirus infection in newborn: A protocol for systematic review and meta-analysis.
机构信息
Yuhuan People's Hospital, Taizhou, China.
Ruian People's Hospital, Wenzhou, China.
出版信息
Medicine (Baltimore). 2022 Nov 25;101(47):e31776. doi: 10.1097/MD.0000000000031776.
BACKGROUND
This study aimed to assess the diagnostic accuracy of polymerase-chain-reaction by saliva.
METHOD
We searched Pubmed, EMBASE, Cochrane Library and Web of Science. A case-control or cohort study designed to explore the saliva specimens for diagnosing the cytomegalovirus infection was eligible for this study. Sensitivity (SEN), specificity (SPE), diagnostic odds ratio (DOR), and summary receiver operating characteristic curves were calculated.
RESULTS
Nine articles were selected for the meta-analysis. For assessing saliva PCR with urine culture, the SEN, SPE, and DOR were 0.97 [95% confidential interval (CI):0.61,1.00] 1.00 [95%CI:0.99, 1.00] 15672 [95%CI:558, 440325], respectively; for assessing saliva PCR with urine PCR, the SEN, SPE, and DOR were 0.87 [95%CI:0.79, 0.92] 1.00 [95%CI:1.00, 1.00] 15637 [95%CI:5946, 41126], respectively; for total assessment, the SEN, SPE, and DOR were0.91 [95%CI:0.70, 0.98] 1.00 [95%CI:1.00, 1.00] 8493 [95%CI:1429, 50487], respectively.
CONCLUSION
We reported that saliva PCR performed well in the diagnosis of cytomegalovirus infection.
背景
本研究旨在评估唾液聚合酶链反应的诊断准确性。
方法
我们检索了 Pubmed、EMBASE、Cochrane 图书馆和 Web of Science。本研究纳入了旨在探索唾液标本用于诊断巨细胞病毒感染的病例对照或队列研究。计算了灵敏度（SEN）、特异性（SPE）、诊断比值比（DOR）和汇总受试者工作特征曲线。
结果
有 9 篇文章被纳入荟萃分析。对于评估唾液 PCR 与尿液培养，SEN、SPE 和 DOR 分别为 0.97[95%置信区间（CI）：0.61，1.00]、1.00[95%CI：0.99，1.00]、15672[95%CI：558，440325]；对于评估唾液 PCR 与尿液 PCR，SEN、SPE 和 DOR 分别为 0.87[95%CI：0.79，0.92]、1.00[95%CI：1.00，1.00]、15637[95%CI：5946，41126]；总评估中，SEN、SPE 和 DOR 分别为 0.91[95%CI：0.70，0.98]、1.00[95%CI：1.00，1.00]、8493[95%CI：1429，50487]。
结论
我们报告唾液 PCR 在巨细胞病毒感染的诊断中表现良好。
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