Tang Lipeng, Bai Xue, Xie Xiaohong, Chen Guanhe, Jia Xianbo, Lei Ming, Li Congyan, Lai Songjia
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Sichuan Provincial Key Laboratory of Animal Genetics and Breeding, Sichuan Academy of Animal Science, Chengdu, China.
Front Vet Sci. 2022 Nov 14;9:1009182. doi: 10.3389/fvets.2022.1009182. eCollection 2022.
Numerous studies have highlighted the role of miRNA in the deformation and necrosis of cells of ovarian tissue caused by heat stress (HS), which ultimately affects ovarian function. Although the role of small RNAs has been investigated in alterations in ovarian tissue functioning in response to HS, the expression profile of ovarian miRNA has been explored to a lesser extent. In this study, female rabbits were subject to HS treatment by using electrical heater. The current work demonstrated that HS could significantly change physiological performance of female rabbits including body weight, rectal temperature and relative ovary weight, and significantly reduce serum , and concentrations by enzyme-linked immunosorbent assay (ELISA) technique. As a result, an increase in apoptosis in ovarian cells, as well as unhealthy follicles, were observed by Hematoxylin-eosin (HE) and TUNEL staining. Additionally, small RNA-seq revealed changes in the miRNA expression profile of rabbit ovaries under HS. Five hundred fourteen miRNAs were obtained including known miRNAs 442 and novel miRNAs 72. Among these miRNAs, 23 miRNAs were significantly expressed under HS. Eleven differentially expressed miRNAs (DE miRNAs) and 9 their predicted targets were confirmed by qPCR, which were expected miRNA-mRNA negative regulation pattern. Among the DE miRNAs and targets, miR-141-39 may target , miR-449a-5p and miR-34c-5p may control and together, miR-449a-5p may target , miR-34c-5p potentially targets and miR-196b-5p potentially regulates and . Thus, the current work suggested the negative effects of HS on the ovarian tissue of female rabbits, and in conclusion these changes could be caused by decreased serum and levels, increased ovarian apoptosis, and changed the expression of miRNAs.
大量研究强调了微小RNA（miRNA）在热应激（HS）引起的卵巢组织细胞变形和坏死中的作用，这最终会影响卵巢功能。尽管已经研究了小RNA在卵巢组织功能因热应激而发生改变中的作用，但对卵巢miRNA表达谱的探索程度较低。在本研究中，使用电加热器对雌性兔子进行热应激处理。当前的研究表明，热应激可显著改变雌性兔子的生理性能，包括体重、直肠温度和相对卵巢重量，并通过酶联免疫吸附测定（ELISA）技术显著降低血清[具体物质1]、[具体物质2]和[具体物质3]的浓度。结果，通过苏木精-伊红（HE）染色和TUNEL染色观察到卵巢细胞凋亡增加以及不健康卵泡的出现。此外，小RNA测序揭示了热应激下兔子卵巢miRNA表达谱的变化。共获得514个miRNA，包括已知的miR-442和新的miR-72。在这些miRNA中，有23个miRNA在热应激下显著表达。通过qPCR证实了11个差异表达的miRNA（DE miRNA）及其9个预测靶点，预期为miRNA- mRNA负调控模式。在这些DE miRNA及其靶点中，miR-141-39可能靶向[具体基因1]，miR-449a-5p和miR-34c-5p可能共同调控[具体基因2]和[具体基因3]，miR-449a-5p可能靶向[具体基因4]，miR-34c-5p可能潜在靶向[具体基因5]，miR-196b-5p可能潜在调控[具体基因6]和[具体基因7]。因此，当前的研究表明热应激对雌性兔子的卵巢组织有负面影响，总之，这些变化可能是由于血清[具体物质1]和[具体物质2]水平降低、卵巢细胞凋亡增加以及miRNA表达改变所致。
