Odukoya L A, Badmos K B, Khramtsova G, Adebayo L A, Olopade O I, Abdulkareem F B
Department of Anatomic and Molecular Pathology, Lagos University Teaching Hospital, Lagos State, Nigeria.
Department of Anatomic and Molecular Pathology, College of Medicine, University of Lagos and Lagos University Teaching Hospital, Lagos State, Nigeria.
West Afr J Med. 2022 Nov 30;39(11):1134-1140.
Colorectal cancer (CRC) is the fourth most common cancer in Nigeria, and it affects mostly persons in their middle age. In a bid to gain some insight into the molecular characteristics of CRC in our environment, we set out to investigate the expression of COX-2 and HER-2 among Nigerian subjects.
To evaluate the expression of COX-2 and HER-2 and determine their correlation with clinicopathologic parameters in surgically resected histologically diagnosed cases of colorectal cancer.
Fifty-three paraffin-embedded tissue blocks of colorectal resections and corresponding patient information were retrieved from the archives of the Anatomic and Molecular Pathology Department of Lagos University Teaching Hospital. A 4-micron slide section was obtained from each specimen and immunohistochemistry for COX-2 and HER-2 expression was performed.
Mean age of cases was 53.9years with an almost equal M:F ratio of 1.12:1. Half of the cases were moderately differentiated adenocarcinoma and 17% were high grade tumors. Eighty three percent of the tumours showed positive cytoplasmic COX-2 expression and extremely low membranous HER-2 positivity was observed in 2%. There was no significant correlation between COX-2 expression and age, gender, tumour location, tumour size, depth of invasion or lymph node status. However, COX-2 expression revealed a significant correlation with tumour grade (p= 0.013).
This study detects a high COX-2 and low HER- 2 expression in colorectal cancer using immunohistochemistry, suggesting a possible role for COX-2 in CRC pathogenesis. This report should trigger further investigations of both markers vis-à-vis the management of CRC in our environment.
结直肠癌（CRC）是尼日利亚第四大常见癌症，主要影响中年人群。为了深入了解我国环境中结直肠癌的分子特征，我们着手调查尼日利亚受试者中COX-2和HER-2的表达情况。
评估COX-2和HER-2的表达，并确定它们与手术切除的组织学诊断结直肠癌病例的临床病理参数之间的相关性。
从拉各斯大学教学医院解剖与分子病理科档案中检索出53个结直肠癌手术切除的石蜡包埋组织块及相应的患者信息。从每个标本中获取4微米厚的切片，并进行COX-2和HER-2表达的免疫组织化学检测。
病例的平均年龄为53.9岁，男女比例几乎相等，为1.12:1。一半的病例为中度分化腺癌，17%为高级别肿瘤。83%的肿瘤显示细胞质COX-2表达阳性，2%的肿瘤观察到极低的膜HER-2阳性。COX-2表达与年龄、性别、肿瘤位置、肿瘤大小、浸润深度或淋巴结状态之间无显著相关性。然而，COX-2表达与肿瘤分级显示出显著相关性（p = 0.013）。
本研究通过免疫组织化学检测发现结直肠癌中COX-2表达高而HER-2表达低，提示COX-2在结直肠癌发病机制中可能起作用。本报告应引发对这两种标志物在我国环境中结直肠癌管理方面的进一步研究。
