Wang Jin-Hai, Du Xiao-Zheng, Yuan Bo, Li Xing-Lan, Peng Xiao-Yun, Jiang Hua
Department of Traditional Chinese Medicine, the Second Hospital of Lanzhou University, Lanzhou 730030, China.
Academy of Acupuncture and Massage, Gansu University of Chinese Medicine, Lanzhou 730000.
Zhen Ci Yan Jiu. 2022 Nov 25;47(11):949-54. doi: 10.13702/j.1000-0607.20211061.
To observe the effect of scalp acupuncture on the expression of argarginine vasopressin receptor-1a(VR), phosphorylated calmodulin-dependent protein kinase Ⅱ(p-CaMKⅡ), and aquaporin 4(AQP) at hypothalamus in middle cerebral artery occlusion (MCAO) rats, so as to explore the molecular mechanisms of scalp acupuncture reducing encepha-ledema in acute ischemic stroke.
A total of 96 male SD rats were randomly divided into normal， model, inhibitor and scalp acupuncture groups, with 24 rats in each group. The MCAO model was established by thread occlusion method. The inhibitor group was intraperitoneally injected with VR inhibitor (30 μg/kg)，once a day for 7 consecutive days. In the scalp acupuncture group, acupuncture was applied to bilateral "parietal and temporal anterior oblique line", with rapid insertion of 2 needles at 15° to 20°, twisting at 100 r/min for 1 min, and retaining the needles for 30 min, once a day for 7 consecutive days. The neurologic deficit score (NDS) and neurological score (NS) were evaluated before and after intervention. The positive expression of p-CaMKⅡ and AQP proteins in hypothalamus was detected by immunohistochemistry. The water content of left brain tissue was determined by BIIiot method. The expression of VR mRNA in hypothalamus was detected by real-time PCR.
Compared with the normal group, the NDS, NS, hypothalamic VR mRNA expression, water content of the brain tissue, and hypothalamic p-CaMKⅡ and AQP positive expression levels were significantly increased (<0.01) in the model group. Compared with the model group, the NDS, NS, hypothalamic VR mRNA expression, water content of the brain tissue, and hypothalamic p-CaMKⅡ and AQP positive expression levels were significantly decreased (<0.01) in the inhibitor and scalp acupuncture groups.
Regulating the signaling pathway of VR/CaMKⅡ/AQP in hypothalamus may be one of the molecular mechanisms of scalp acupuncture reducing encephaledema in acute ischemic stroke.
观察头针疗法对大脑中动脉闭塞（MCAO）大鼠下丘脑精氨酸加压素受体 -1a（VR）、磷酸化钙调蛋白依赖性蛋白激酶Ⅱ（p-CaMKⅡ）和水通道蛋白4（AQP）表达的影响，以探讨头针减轻急性缺血性脑卒中脑水肿的分子机制。
将96只雄性SD大鼠随机分为正常组、模型组、抑制剂组和头针组，每组24只。采用线栓法建立MCAO模型。抑制剂组腹腔注射VR抑制剂（30μg/kg），每天1次，连续7天。头针组针刺双侧“顶颞前斜线”，进针角度15°～20°，快速刺入2针，以100r/min捻转1min，留针30min，每天1次，连续7天。干预前后评估神经功能缺损评分（NDS）和神经评分（NS）。采用免疫组织化学法检测下丘脑p-CaMKⅡ和AQP蛋白的阳性表达。采用干湿重法测定左侧脑组织含水量。采用实时荧光定量PCR法检测下丘脑VR mRNA表达。
与正常组比较，模型组NDS、NS、下丘脑VR mRNA表达、脑组织含水量及下丘脑p-CaMKⅡ和AQP阳性表达水平均显著升高（<0.01）。与模型组比较，抑制剂组和头针组NDS、NS、下丘脑VR mRNA表达、脑组织含水量及下丘脑p-CaMKⅡ和AQP阳性表达水平均显著降低（<0.01）。
调节下丘脑VR/CaMKⅡ/AQP信号通路可能是头针减轻急性缺血性脑卒中脑水肿的分子机制之一。
