Ma Xue, Wang Qiang, Wang Yuan, Yuan Wei, Liu Zhi-Bin, Qiao Hai-Fa
College of Acupuncture and Moxibustion, Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi Province, China; Shaanxi Key Laboratory of Acupuncture and Herbal Medicine, Xianyang 712046, Shaanxi Province, China; Shaanxi Provincial Key Laboratory of Traditional Chinese Medicine Encephalopathy, Xianyang 712046, Shaanxi Province, China.
Zhen Ci Yan Jiu. 2022 Nov 25;47(11):993-8. doi: 10.13702/j.1000-0607.20211111.
To observe the effect of early electroacupuncture(EA) intervention on ionized calcium binding adapter molecule 1 (Iba-1), tyrosine hydroxylase (TH) and tumor necrosis factor-α (TNF-α) in Parkinson's disease (PD) mice, so as to explore its neuroinflammation mechanism in treating PD.
A total of 24 male C57BL/6J mice (9 weeks old) were randomly divided into control, model and EA groups, with 8 mice in each group. The PD model was established by long-term low dose subcutaneous injection of rotenone. Started at the same time with modeling, EA (2 Hz/100 Hz, 1 mA) was applied to "Shenting"(GV24), bilateral "Tianshu"(LI11), "Quchi"(ST25), and "Shangjuxu"(ST37) for 15 min, once a day for 8 weeks. The motor function was assessed by rotorod test and step length test. The expression levels of Iba-1 and TH proteins in substantia nigra pars compacta (SNpc) was detected by Western blot and immunohistochemistry. The expression level of TNF-α protein in colon tissue was examined by Western blot and immunofluorescence staining.
Compared with the control group, the fall latency shortened at 4, 6, and 8 weeks after modeling (<0.01) and the step length of hind limbs shortened at 5 and 7 weeks after modeling (<0.01), the expression levels of Iba-1 in SNpc and TNF-α in colon tissue were increased (<0.01), and the expression level of TH in SNpc was decreased (<0.01) in the model group. Compared with the model group, the fall latency prolonged at 6 and 8 weeks after modeling (<0.01) and the step length of hind limbs prolonged at 5 and 7 weeks after modeling (<0.01), the expression levels of Iba-1 in SNpc and TNF-α in colon tissue were decreased (<0.01, <0.05), and the expression level of TH in SNpc was increased (<0.05, <0.01) in the EA group.
Early EA intervention can delay the occurring time of motor disfunction, alleviated the loss of substantia nigra dopaminergic neurons, and exerted a good neuroprotective effect on the degenerative changes in rotenone-induced PD mice, which may be related to its effects in alleviating the intestinal inflammation, inhibiting the activation of microglia, thereby reducing the neuroinflammation.
观察早期电针干预对帕金森病（PD）小鼠离子钙结合衔接分子1（Iba-1）、酪氨酸羟化酶（TH）和肿瘤坏死因子-α（TNF-α）的影响，以探讨其治疗PD的神经炎症机制。
将24只9周龄雄性C57BL/6J小鼠随机分为对照组、模型组和电针组，每组8只。通过长期低剂量皮下注射鱼藤酮建立PD模型。与建模同时开始，将电针（2Hz/100Hz，1mA）应用于“神庭”（GV24）、双侧“天枢”（LI11）、“曲池”（ST25）和“上巨虚”（ST37），每次15分钟，每天1次，共8周。通过转棒试验和步长试验评估运动功能。采用蛋白质免疫印迹法和免疫组织化学法检测黑质致密部（SNpc）中Iba-1和TH蛋白的表达水平。采用蛋白质免疫印迹法和免疫荧光染色法检测结肠组织中TNF-α蛋白的表达水平。
与对照组相比，模型组建模后4、6、8周转棒潜伏期缩短（<0.01），建模后5、7周后肢步长缩短（<0.01），SNpc中Iba-1和结肠组织中TNF-α的表达水平升高（<0.01），SNpc中TH的表达水平降低（<0.01）。与模型组相比，电针组建模后6、8周转棒潜伏期延长（<0.01），建模后5、7周后肢步长延长（<0.01），SNpc中Iba-1和结肠组织中TNF-α的表达水平降低（<0.01，<0.05），SNpc中TH的表达水平升高（<0.05，<0.01）。
早期电针干预可延缓运动功能障碍的发生时间，减轻黑质多巴胺能神经元的丢失，对鱼藤酮诱导的PD小鼠的退行性变具有良好的神经保护作用，这可能与其减轻肠道炎症、抑制小胶质细胞激活从而减轻神经炎症的作用有关。
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