[电针干预对帕金森病大鼠黑质中ERK 1/2信号通路及TNF-α和IL-1β蛋白水平的影响]

[Effect of electroacupuncture intervention on ERK 1/2 signaling and TNF-α and IL-1β protein levels in the substantia Nigra in rats with Parkinson's Disease].

作者信息

Wang Shu-ju, Ma Jun, Gong Yuan-xun, Wang Yan-chun, Zeng Xiao-ling, Liang Yan, Sun Guo-jie

出版信息

Zhen Ci Yan Jiu. 2014 Dec;39(6):456-60.

Abstract

OBJECTIVE

To observe the effect of electroacupuncture(EA) on phosphalized extracellular regulated protein kinases(p-ERK) 1/2 signaling pathway and tumor necrosis factor (TNF)-α and IL-1β protein levels in the substantia nigra (SN) in rats with Parkinson's disease (PD), so as to explore its mechanism underlying improvement of PD.

METHODS

32 male SD rats were randomly and averagely divided into normal control group, sham-operation group, model group and EA group. PD model was established by intra-dermal-injection of rotenone(1 mg/kg, dissolved in DMSO and saline, concentration: 0. 25 mg/mL) at the nape, once daily for 14 days. EA (2 Hz, 2 mA) was applied to "Fengfu"(GV 16)and "Taichong"(LR 3) for 20 min, once daily for 14 days. For sham-operation group, subcutaneous injection of the same dose of DMSO and saline was given in the same way. The expression levels of tyrosine hydroxylase (TH), p-ERK 1/2, inflammatory cytokines TNF-α and IL-1P proteins of the SN tissue were detected using Western blot. The rat's horizontal and vertical movement ability was assessed using open-field tests.

RESULTS

The horizontal and vertical movement scores were significantly lower in the model group than in the normal control group, and markedly higher in the EA group than in the model group (P<0. 05). Compared with the normal control group, the expression level of TH protein in the SN was significantly reduced in the model group( P<0. 05 ),while the expression levels of p-ERK 1/2, TNF-α and IL-1β proteins were significantly increased in the model group(P<0. 05). After EA intervention, the expression level of TH protein was significantly increased(P<0.05),and those of p-ERK 1/2, TNF-α and IL-1β proteins were significantly down-regulated(P<0. 05) in the EA group compared to the model group.

CONCLUSION

EA therapy may improve PD rats' movement ability, which may be associated with its effects in down-regulating the expression levels of p-ERK 1/2, TNF-α and IL-1β proteins and up-regulating the expression of TH protein in the SN.

摘要

目的

观察电针对帕金森病（PD）大鼠黑质中磷酸化细胞外调节蛋白激酶（p-ERK）1/2信号通路及肿瘤坏死因子（TNF）-α和白细胞介素-1β蛋白水平的影响，以探讨其改善PD的作用机制。

方法

将32只雄性SD大鼠随机平均分为正常对照组、假手术组、模型组和电针组。采用皮下注射鱼藤酮（1mg/kg，溶于二甲基亚砜和生理盐水中，浓度：0.25mg/mL）于大鼠颈部建立PD模型，每天1次，连续14天。电针组针刺“风府”（GV 16）和“太冲”（LR 3），频率2Hz，强度2mA，留针20min，每天1次，连续14天。假手术组以同样方式皮下注射相同剂量的二甲基亚砜和生理盐水。采用蛋白质免疫印迹法检测黑质组织中酪氨酸羟化酶（TH）、p-ERK 1/2、炎性细胞因子TNF-α和IL-1β蛋白的表达水平。采用旷场试验评估大鼠的水平和垂直运动能力。

结果

模型组大鼠的水平和垂直运动评分显著低于正常对照组，电针组显著高于模型组（P<0.05）。与正常对照组比较，模型组黑质TH蛋白表达水平显著降低（P<0.05），p-ERK 1/2、TNF-α和IL-1β蛋白表达水平显著升高（P<0.05）。电针干预后，电针组黑质TH蛋白表达水平显著升高（P<0.05），p-ERK 1/2、TNF-α和IL-1β蛋白表达水平显著下调（P<0.05）。