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线粒体 GPAT 衍生的 LPA 控制生长素依赖性胚胎和胚胎后发育。

Mitochondrial GPAT-derived LPA controls auxin-dependent embryonic and postembryonic development.

机构信息

State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.

Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, China.

出版信息

Proc Natl Acad Sci U S A. 2022 Dec 6;119(49):e2212881119. doi: 10.1073/pnas.2212881119. Epub 2022 Dec 1.

DOI:10.1073/pnas.2212881119
PMID:36454754
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9894262/
Abstract

Membrane properties are emerging as important cues for the spatiotemporal regulation of hormone signaling. Lysophosphatidic acid (LPA) evokes multiple biological responses by activating G protein-coupled receptors in mammals. In this study, we demonstrated that LPA derived from the mitochondrial glycerol-3-phosphate acyltransferases GPAT1 and GPAT2 is a critical lipid-based cue for auxin-controlled embryogenesis and plant growth in . LPA levels decreased, and the polarity of the auxin efflux carrier PIN-FORMED1 (PIN1) at the plasma membrane (PM) was defective in the mutant. As a consequence of distribution defects, instructive auxin gradients and embryonic and postembryonic development are severely compromised. Further cellular and genetic analyses revealed that LPA binds directly to PIN1, facilitating the vesicular trafficking of PIN1 and polar auxin transport. Our data support a model in which LPA provides a lipid landmark that specifies membrane identity and cell polarity, revealing an unrecognized aspect of phospholipid patterns connecting hormone signaling with development.

摘要

膜特性正成为激素信号时空调节的重要线索。溶血磷脂酸（LPA）通过激活哺乳动物中的 G 蛋白偶联受体，引发多种生物学反应。在这项研究中，我们表明，来源于线粒体甘油-3-磷酸酰基转移酶 GPAT1 和 GPAT2 的 LPA 是生长素控制胚胎发生和植物生长的关键脂质信号。LPA 水平降低，质膜（PM）中生长素外排载体 PIN 形成蛋白 1（PIN1）的极性在 突变体中出现缺陷。由于分布缺陷，指导生长素梯度和胚胎及胚胎后发育严重受损。进一步的细胞和遗传分析表明，LPA 直接与 PIN1 结合，促进 PIN1 的囊泡运输和极性生长素运输。我们的数据支持这样一种模型，即 LPA 提供了一个脂质标志，指定了膜特性和细胞极性，揭示了连接激素信号与发育的磷脂模式的一个未被认识的方面。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c43/9894262/f4f972daa4b6/pnas.2212881119fig07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c43/9894262/fbe333bf776e/pnas.2212881119fig01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c43/9894262/2b68f4e4b0f5/pnas.2212881119fig02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c43/9894262/c84392729e70/pnas.2212881119fig03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c43/9894262/7a7178c46370/pnas.2212881119fig04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c43/9894262/a557edf726ec/pnas.2212881119fig05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c43/9894262/bafbca11da37/pnas.2212881119fig06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c43/9894262/f4f972daa4b6/pnas.2212881119fig07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c43/9894262/fbe333bf776e/pnas.2212881119fig01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c43/9894262/2b68f4e4b0f5/pnas.2212881119fig02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c43/9894262/c84392729e70/pnas.2212881119fig03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c43/9894262/7a7178c46370/pnas.2212881119fig04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c43/9894262/a557edf726ec/pnas.2212881119fig05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c43/9894262/bafbca11da37/pnas.2212881119fig06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c43/9894262/f4f972daa4b6/pnas.2212881119fig07.jpg

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