College of tobacco Science, Henan agricultural university, National tobacco cultivation and physiology and Biochemistry Research center, Key laboratory for tobacco cultivation of tobacco industry, Zhengzhou, Henan, China.
College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China.
Ecotoxicol Environ Saf. 2022 Dec 15;248:114312. doi: 10.1016/j.ecoenv.2022.114312. Epub 2022 Nov 29.
Chromium (Cr) is a harmful heavy metal that poses a serious threat to plants and animals. Selenium (Se) and molybdenum (Mo) are two beneficial elements for plant growth and resistance. However, their interactive effects on Cr uptake and distribution are poorly understood. Therefore, a hydroponics experiment was conducted to explore the effects of the use of Se and Mo alone and simultaneously on mitigating Cr toxicity. In this study, Nicotiana tabacum L. seedlings were exposed to control, 50 µM Cr, 50 μM Cr + 2 μM Se, 50 μM Cr + 1 μM Mo, or 50 μM Cr + 2 μM Se + 1 μM Mo in Hoagland solution. After 2 weeks, the plant biomass, Cr, Se and Mo contents, photosynthesis, leaf ultrastructure, antioxidant system, subcellular distribution and associated gene expression in Nicotiana tabacum L. were determined. The results showed that simultaneous use of Se and Mo promoted tobacco growth under Cr stress, as evidenced by reducing reactive oxygen species (ROS) content and reducing Cr translocation factor (TF) and inducing a 51.3% reduction in Cr content in shoots. Additionally, Se-Mo interactions increased the levels of glutathione (GSH) and phytochelatin (PC) and the distribution of Cr in the cell walls and organelles. Furthermore, the relative expression of PCS1 was upregulated, while those of NtST1 and MSN1 were downregulated. The results concluded that the simultaneous use of Se and Mo effectively alleviated Cr toxicity in Nicotiana tabacum L., which not only offers an efficient way for crops to resist Cr toxicity but also provides evidence for the benefit of Se combined with Mo.
铬（Cr）是一种有害的重金属，对动植物构成严重威胁。硒（Se）和钼（Mo）是两种有益于植物生长和抗性的元素。然而，它们对 Cr 吸收和分布的相互作用知之甚少。因此，进行了水培实验以探索单独和同时使用 Se 和 Mo 减轻 Cr 毒性的效果。在这项研究中，将烟草幼苗暴露于对照、50µM Cr、50µM Cr+2µM Se、50µM Cr+1µM Mo 或 50µM Cr+2µM Se+1µM Mo 在 Hoagland 溶液中。2 周后，测定了烟草的生物量、Cr、Se 和 Mo 含量、光合作用、叶片超微结构、抗氧化系统、亚细胞分布和相关基因表达。结果表明，同时使用 Se 和 Mo 促进了 Cr 胁迫下烟草的生长，这表现为降低活性氧（ROS）含量和降低 Cr 转移因子（TF），并将 Cr 含量在地上部分的含量降低了 51.3%。此外，Se-Mo 相互作用增加了谷胱甘肽（GSH）和植物螯合肽（PC）的水平以及 Cr 在细胞壁和细胞器中的分布。此外，PCS1 的相对表达上调，而 NtST1 和 MSN1 的相对表达下调。研究结果表明，同时使用 Se 和 Mo 有效缓解了烟草中的 Cr 毒性，不仅为作物抵抗 Cr 毒性提供了有效途径，而且为 Se 与 Mo 结合带来的益处提供了证据。
