College of Animal Science and Technology, Gansu Agricultural University, No. 1, Yingmen Village, Anning District, Lanzhou City, Gansu Province 730070, PR China.
College of Animal Science and Technology, Gansu Agricultural University, No. 1, Yingmen Village, Anning District, Lanzhou City, Gansu Province 730070, PR China.
Comp Biochem Physiol Part D Genomics Proteomics. 2023 Mar;45:101042. doi: 10.1016/j.cbd.2022.101042. Epub 2022 Nov 24.
Alternative splicing (AS) is a ubiquitous post-transcriptional regulatory mechanism in eukaryotes that generates multiple mRNA isoforms from a single gene, increasing diversity of mRNAs and proteins that are essential for eukaryotic developmental processes and responses to environmental stress. Results showed that a total of 37,463 AS events were identified in rainbow trout hepatocytes. In addition, a total of 364 differential alternative splicing (DAS) events were identified in hepatocytes under selenium nanoparticles (SeNPs) and 3632 DAS events were identified under a combination of SeNPs and heat stress (24 °C). Gene Ontology (GO) enrichment showed that some subcategories "immune effector processes", "response to stimuli" and "antioxidant activity" were associated with immunity, abiotic stimuli and antioxidants. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment showed that differentially expressed genes (DEGs) were significantly enriched in spliceosomes by adding SeNPs in heat-stressed hepatocytes. Splicing factor family (SRSF3, SRSF7, SRSF9, U2AF1 and U2AF2) and pre-RNA splicing factors (ACIN1 and PPRF18) were significantly upregulated and promoted AS. Furthermore, addition of SeNPs activated the phosphatidylinositol signaling system and upregulated the related genes PI4KA, DGKH, ITPK1 and Ocrl, and thus attenuated the inflammatory response to heat stress and enhanced resistance to heat stress by activating the adherent plaque kinase-PI3K-Akt signaling pathway and calcium channels. Those findings suggested that AS could be an essential regulatory mechanism in adaptation of rainbow trout to heat-stressed environments.
可变剪接（AS）是真核生物中普遍存在的转录后调控机制，它从单个基因产生多种 mRNA 异构体，增加了 mRNA 和蛋白质的多样性，这些多样性对于真核生物的发育过程和对环境压力的反应至关重要。结果表明，在虹鳟鱼肝细胞中鉴定出了总共 37463 个 AS 事件。此外，在硒纳米颗粒（SeNPs）和 SeNPs 与热应激（24°C）联合作用下，肝细胞中鉴定出总共 364 个差异可变剪接（DAS）事件和 3632 个 DAS 事件。基因本体论（GO）富集显示，一些亚类“免疫效应子过程”、“对刺激的反应”和“抗氧化活性”与免疫、非生物刺激和抗氧化剂有关。京都基因与基因组百科全书（KEGG）富集显示，在热应激的肝细胞中添加 SeNPs 后，差异表达基因（DEGs）在剪接体中显著富集。剪接因子家族（SRSF3、SRSF7、SRSF9、U2AF1 和 U2AF2）和前 RNA 剪接因子（ACIN1 和 PPRF18）显著上调并促进 AS。此外，添加 SeNPs 激活了磷脂酰肌醇信号系统，上调了相关基因 PI4KA、DGKH、ITPK1 和 Ocrl，从而通过激活粘着斑激酶-PI3K-Akt 信号通路和钙通道减轻了对热应激的炎症反应，增强了对热应激的抵抗力。这些发现表明，AS 可能是虹鳟鱼适应热应激环境的重要调控机制。
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