Zhao Xianxian, Wang Yizhou, Wang Zhenlu, Luo Tianma, Huang Jun, Shao Jian
College of Animal Science, Guizhou University, Guiyang 550025, China.
Key Laboratory of Animal Diseases and Veterinary Public Health in Guizhou Province, Guiyang 550025, China.
Animals (Basel). 2024 Oct 17;14(20):3005. doi: 10.3390/ani14203005.
Temperature is one of the critical factors affecting the physiological functions of fish. With ongoing global warming, changes in water temperature have a profound impact on fish species. Alternative splicing, being a significant mechanism for gene expression regulation, facilitates fish to adapt and thrive in dynamic and varied aquatic environments. Our study used transcriptome sequencing to analyze alternative splicing in largemouth bass gills at 34 °C for 24 h. The findings indicated an increase in both alternative splicing events and alternative splicing genes after high temperature treatment. Specifically, the comparative analysis revealed a total of 674 differential alternative splicing events and 517 differential alternative splicing genes. Enrichment analysis of differential alternative splicing genes revealed significant associations with various gene ontology (GO) terms and KEGG pathways, particularly in immune-related pathways like necroptosis, apoptosis, and the C-type lectin receptor signaling pathway. These results emphasize that some RNA splicing-related genes are involved in the response of largemouth bass to high temperatures.
温度是影响鱼类生理功能的关键因素之一。随着全球变暖的持续，水温变化对鱼类物种产生了深远影响。可变剪接作为基因表达调控的重要机制，有助于鱼类在动态多变的水生环境中适应并茁壮成长。我们的研究利用转录组测序分析了大口黑鲈鳃在34℃处理24小时后的可变剪接情况。研究结果表明，高温处理后可变剪接事件和可变剪接基因均有所增加。具体而言，比较分析共发现674个差异可变剪接事件和517个差异可变剪接基因。对差异可变剪接基因的富集分析揭示了与各种基因本体（GO）术语和KEGG通路的显著关联，特别是在坏死性凋亡、凋亡和C型凝集素受体信号通路等免疫相关通路中。这些结果强调了一些与RNA剪接相关的基因参与了大口黑鲈对高温的反应。
