外胚层间充质干细胞通过靶向 sonic hedgehog 信号通路促进 MC3T3-E1 细胞的成骨分化。
Ectoderm mesenchymal stem cells promote osteogenic differentiation of MC3T3-E1 cells by targeting sonic hedgehog signaling pathway.
机构信息
Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu Province, China.
Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu Province, China.
出版信息
Mol Biol Rep. 2023 Feb;50(2):1293-1302. doi: 10.1007/s11033-022-08022-8. Epub 2022 Dec 1.
BACKGROUND
Despite their high repair capability, bone defects still present a major challenge in orthopedic tissue engineering. Osteoblast differentiation is central to the treatment of bone defects. METHODS AND RESULTS: We used nasal mucosal-derived ectoderm mesenchymal stem cells (EMSCs) to promote osteogenic differentiation by co-culturing MC3T3-E1 cells. Our results showed that MC3T3-E1/EMSCs co-culture upregulated bone-related proteins and transglutaminase 2 (TG2) and increased alkaline phosphatase (ALP) activity and bone nodule formation relative to controls. Furthermore, our results showed that EMSC-derived sonic hedgehog (Shh) accounted for the enhanced MC3T3-E1 differentiation because inhibiting Shh signaling substantially reduced osteogenic differentiation.
CONCLUSION
Altogether, these results suggest that EMSCs differentiated into osteoblast cells and supported MC3T3-E1 differentiation. Thus, EMSCs may be a promising cell source for treating bone-related diseases.
背景
尽管具有较高的修复能力，但骨缺损仍然是骨科组织工程中的主要挑战。成骨细胞分化是治疗骨缺损的核心。方法与结果：我们使用鼻黏膜衍生外胚层间充质干细胞（EMSCs）通过与 MC3T3-E1 细胞共培养来促进成骨分化。结果表明，与对照组相比，MC3T3-E1/EMSCs 共培养上调了骨相关蛋白和转谷氨酰胺酶 2（TG2）的表达，并增加了碱性磷酸酶（ALP）活性和骨结节形成。此外，我们的结果表明，EMSC 衍生的 Sonic Hedgehog（Shh）是促进 MC3T3-E1 分化的原因，因为抑制 Shh 信号通路会显著降低成骨分化。结论：综上所述，这些结果表明，EMSCs 可分化为成骨细胞并支持 MC3T3-E1 的分化。因此，EMSCs 可能是治疗与骨相关疾病的有前途的细胞来源。
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