Taghipour Tayebeh, Rasti Sima, Saba Mohammadali, Delavari Mahdi, Moosavi Gholam Abbas, Hooshyar Hossein, Eslamirad Zahra
Department of Parasitology and Mycology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Department of Internal Medicine, Faculty of Medicine, Shahid Beheshti Hospital, Kashan University of Medical Sciences, Kashan, Iran.
J Parasit Dis. 2022 Dec;46(4):1028-1035. doi: 10.1007/s12639-022-01524-z. Epub 2022 Aug 18.
spp. are the most common free-living amoeba worldwide, inducing life-threatening diseases such as Granulomatous Amoebic Encephalitis, pulmonary infection, and amoebic keratitis. This study aimed to identify the FLA and genotypes in patients with pulmonary symptoms suspected of cancer in Kashan's hospitals, Kashan, Iran. This cross-sectional study was conducted on 97 bronchoalveolar lavage samples of patients with respiratory symptoms suspected of lung cancer, who were admitted to the Shahid Beheshti Hospital of Kashan from 2019 to 2020. The samples were cultured onto 1.5% non-nutrient agar enriched with killed and examined for the presence of FLA. Following amoeba isolation and DNA extraction, . were determined by Polymerase Chain Reaction using JDP1 and JDP2 primers, which amplified a 490 bp fragment from the 18 S rDNA gene. Eighteen isolates were sequenced, and the genotypes were identified. The prevalence of FLA and and the relationship between symptoms and demographic variables were analyzed with SPSS Software version 16. The prevalence rates of FLA and in the BAL samples was 86.6% and 73.2%, respectively. All isolates belonged to the T4 genotype. The most symptoms among -positive patients were dyspnea and cough; however, their difference was not statistically significant. The findings indicated the high prevalence of FLA and in BAL in the population suspected of cancer in Kashan. Since the T4 genotype is a pathogenic genotype of , training health and improving sanitation levels would help to prevent infection.
棘阿米巴属是全球最常见的自由生活阿米巴，可引发危及生命的疾病，如肉芽肿性阿米巴脑炎、肺部感染和阿米巴角膜炎。本研究旨在鉴定伊朗卡尚市卡尚医院疑似患有癌症且有肺部症状患者中的自由生活阿米巴（FLA）及基因型。本横断面研究对2019年至2020年入住卡尚市沙希德·贝赫什提医院的97例疑似肺癌且有呼吸道症状患者的支气管肺泡灌洗样本进行了检测。将样本接种于添加有灭活大肠杆菌的1.5%非营养琼脂上，检测FLA的存在情况。在分离出阿米巴并提取DNA后，使用JDP1和JDP2引物通过聚合酶链反应确定基因型，这对18S rDNA基因的490bp片段进行了扩增。对18株分离株进行测序并鉴定基因型。使用SPSS软件16版分析FLA和基因型的患病率以及症状与人口统计学变量之间的关系。支气管肺泡灌洗样本中FLA和基因型的患病率分别为86.6%和73.2%。所有分离株均属于T4基因型。基因型阳性患者中最常见的症状是呼吸困难和咳嗽；然而，它们之间的差异无统计学意义。研究结果表明，在卡尚疑似癌症人群的支气管肺泡灌洗样本中，FLA和基因型的患病率较高。由于T4基因型是棘阿米巴的致病基因型，培训卫生人员并提高卫生水平将有助于预防感染。
