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微小RNA-21通过调控Ajuba/Isl1轴通路促进骨髓间充质干细胞向心肌样细胞分化。

MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway.

作者信息

Yang Wengang, Zhao Lei, Xue Song, Liu Jidong, Shan Jianggui

机构信息

Department of Cardiovascular Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

出版信息

Arch Med Sci. 2022 Nov 7;18(6):1672-1677. doi: 10.5114/aoms/154956. eCollection 2022.

DOI:10.5114/aoms/154956
PMID:36457985
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9710289/
Abstract

INTRODUCTION

In this study, we aimed to investigate the role of miRNA-21 and the regulating pathway that promotes the differentiation of bone marrow mesenchymal stem cells (BMSCs).

METHODS

We used miR-21-OE, miR-21-KD, ajuba-OE and ajuba-KD plasmids to infect BMSCs. The expression of miRNA-21, ajuba, Isl1 and cTnI was detected by RT-qPCR, WB and immunofluorescence staining in groups.

RESULTS

MiRNA-21 over-expression increased the expression of Isl1, and vice versa. Ajuba over-expression decreased the expression of Isl1, and vice versa. Ajuba negatively regulated the differentiation of BMSCs into cardiomyocyte-like cells.

CONCLUSIONS

MiRNA-21 could regulate differentiation of bone marrow mesenchymal stem cells (BMSCs) to cardiomyocyte-like cells through the ajuba/Isl1 axis pathway.

摘要

引言

在本研究中，我们旨在探究miRNA-21的作用以及促进骨髓间充质干细胞（BMSCs）分化的调控途径。

方法

我们使用miR-21-OE、miR-21-KD、ajuba-OE和ajuba-KD质粒感染BMSCs。通过RT-qPCR、WB和免疫荧光染色检测各组中miRNA-21、ajuba、Isl1和cTnI的表达。

结果

miRNA-21过表达增加了Isl1的表达，反之亦然。ajuba过表达降低了Isl1的表达，反之亦然。ajuba负向调控BMSCs向心肌样细胞的分化。

结论

miRNA-21可通过ajuba/Isl1轴途径调节骨髓间充质干细胞（BMSCs）向心肌样细胞的分化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfe/9710289/58c869e25914/AMS-18-6-154956-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfe/9710289/de6858a155e6/AMS-18-6-154956-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfe/9710289/457ebd0db750/AMS-18-6-154956-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfe/9710289/58c869e25914/AMS-18-6-154956-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfe/9710289/de6858a155e6/AMS-18-6-154956-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfe/9710289/457ebd0db750/AMS-18-6-154956-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfe/9710289/58c869e25914/AMS-18-6-154956-g003.jpg

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