Ge Mengyi, Zhao Wenfeng, Han Yue, Gai Hongwei, Zong Chenghua
School of Chemistry and Materials Science, Jiangsu Normal University, Xuzhou, Jiangsu, China.
Front Chem. 2022 Nov 15;10:1057241. doi: 10.3389/fchem.2022.1057241. eCollection 2022.
This report presents a contactless and robust dielectric microspheres (DMs)-assisted surface enhanced Raman scattering (SERS) enhancement method to improve SERS detection sensitivity detection sensitivity. DMs that could focus and collect light were embedded within the polydimethylsiloxane (PDMS) film to avoid direct contact with the analytical solution and improve detection reliability. The as prepared DMs embedded PDMS (DMs-PDMS) film was integrated with a microfluidic technique to enhance the SERS signal of a liquid substrate. Detection in microfluidic systems can reduce reagent consumption, shorten assay time, and avoid evaporation of the colloid substrate solution. The robustness and potential influencing factors of DMs-PDMS film assisted SERS enhancement (DERS) were evaluated using 4-aminothiophenol (4-ATP) as the Raman probe. The sensing performance of the proposed method toward dipicolinic acid (DPA) was evaluated, and an evident signal intensification was obtained. Remarkably, the DMs-PDMS film can also be implemented on solid substrates. A proof-of-concept experiment was performed by covering the DMs-PDMS film directly over an AgNPs@Si solid substrate wherein a 5.7-fold sensitivity improvement was achieved.
本报告提出了一种非接触式且稳健的介电微球（DMs）辅助表面增强拉曼散射（SERS）增强方法，以提高SERS检测灵敏度。将能够聚焦和收集光的DMs嵌入聚二甲基硅氧烷（PDMS）薄膜中，以避免与分析溶液直接接触并提高检测可靠性。将制备好的嵌入DMs的PDMS（DMs-PDMS）薄膜与微流控技术相结合，以增强液体基质的SERS信号。在微流控系统中进行检测可以减少试剂消耗、缩短检测时间并避免胶体基质溶液蒸发。以4-氨基硫酚（4-ATP）作为拉曼探针，评估了DMs-PDMS薄膜辅助SERS增强（DERS）的稳健性和潜在影响因素。评估了该方法对吡啶二甲酸（DPA）的传感性能，并获得了明显的信号增强。值得注意的是，DMs-PDMS薄膜也可以应用于固体基质。通过将DMs-PDMS薄膜直接覆盖在AgNPs@Si固体基质上进行了概念验证实验，其中灵敏度提高了5.7倍。
