Mukherjee Ayan, Saha Pranab Chandra, Kar Samiran, Guha Pampa, Das Rabi Sankar, Bera Tapas, Guha Samit
Department of Chemistry, Organic Chemistry Section, Jadavpur University, Kolkata, 700032, India.
Department of Chemistry, City College, 102/1 Raja Rammohan Sarani, Kolkata, 700009, India.
Chembiochem. 2023 Feb 1;24(3):e202200641. doi: 10.1002/cbic.202200641. Epub 2022 Dec 28.
We have demonstrated an efficient synthetic route with crystal structures for the construction of acidic pH-triggered visible-to-NIR interchangeable ratiometric fluorescent pH sensors. This bioresponsive probe exhibits pH-sensitive reversible absorption/emission features, low cytotoxicity, a huge 322 nm bathochromic spectral shift with augmented quantum yield from neutral to acidic pH, high sensitivity and selective targeting ability of live-cell lysosomes with ideal pK , off-to-on narrow NIR absorption/fluorescence signals with high molar absorption coefficient at acidic lysosomal lumen, and in-situ live-cell pH-activated ratiometric imaging of lysosomal pH. Selective staining and ratiometric pH imaging in human carcinoma live-cell lysosomes were monitored by dual-channel confocal laser scanning microscope using a pH-activatable organic fluorescent dye comprising a morpholine moiety for lysosome targeting and an acidic pH openable oxazolidine ring. Moreover, real-time tracking of lysosomes, 3D, and multicolor live-cell imaging have been achieved using the synthesized pH-activatable probe.
我们已经展示了一种用于构建酸性pH触发的可见光到近红外可互换比率荧光pH传感器的具有晶体结构的高效合成路线。这种生物响应探针具有pH敏感的可逆吸收/发射特性、低细胞毒性、从中性到酸性pH有322 nm的巨大红移光谱且量子产率增加、对活细胞溶酶体具有高灵敏度和选择性靶向能力且理想的pK、在酸性溶酶体腔内具有高摩尔吸收系数的从关闭到开启的窄近红外吸收/荧光信号以及溶酶体pH的原位活细胞pH激活比率成像。使用包含用于溶酶体靶向的吗啉部分和酸性pH可打开的恶唑烷环的pH可激活有机荧光染料，通过双通道共聚焦激光扫描显微镜监测人癌活细胞溶酶体中的选择性染色和比率pH成像。此外，使用合成的pH可激活探针实现了溶酶体的实时跟踪、三维和多色活细胞成像。
