Asharaf Sumayya, Chakraborty Kajal
Marine Biotechnology Fish Nutrition and Health Division, Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin, 682018, Kerala, India.
Faculty of Marine Sciences, Lakeside Campus, Cochin University of Science and Technology, Cochin, Kerala, India.
Arch Microbiol. 2022 Dec 2;205(1):10. doi: 10.1007/s00203-022-03346-2.
Since the report of the antibiotic with anticancer properties, scientists have been focusing to isolate and characterize novel anti-microbial natural products possessing anticancer activities. The current study describes the production of seaweed-associated heterotrophic Bacillus altitudinis MTCC13046 with potential anticancer properties. The bacterium was screened for its capacity to diminish the cell proliferation of the human hepatocellular adenocarcinoma (HepG2) cell line, without upsetting the normal cells. The bacterial extract showed anticancer properties in a dose-reactive form against HepG2 (IC, half maximal inhibitory concentration ~ 29.5 µg/ml) on tetrazolium bromide analysis with less significant cytotoxicity on common fibroblast (HDF) cells (IC ~ 77 µg/ml). The potential antioxidant ability of the organic extract of B. altitudinis MTCC13046 (IC 133 µg/ml) could corroborate its capacity to attenuate the pathophysiology leading to carcinogenesis. The results of the apoptosis assay showed that the crude extracts of B. altitudinis maintained 68% viability in normal cells compared to 11% in the cancer cells (IC 76.9 µg/ml). According to the findings, B. altitudinis MTCC13046 could be used to develop prospective anticancer agents.
自从具有抗癌特性的抗生素被报道以来，科学家们一直致力于分离和鉴定具有抗癌活性的新型抗菌天然产物。当前的研究描述了具有潜在抗癌特性的与海藻相关的异养型高海拔芽孢杆菌MTCC13046的产生。对该细菌进行了筛选，以评估其在不影响正常细胞的情况下减少人肝细胞腺癌（HepG2）细胞系细胞增殖的能力。在溴化四氮唑分析中，该细菌提取物对HepG2呈现剂量反应形式的抗癌特性（IC，半数最大抑制浓度约为29.5微克/毫升），而对普通成纤维细胞（HDF）细胞的细胞毒性较小（IC约为77微克/毫升）。高海拔芽孢杆菌MTCC13046有机提取物的潜在抗氧化能力（IC为133微克/毫升）可以证实其减轻导致癌症发生的病理生理过程的能力。凋亡分析结果表明，高海拔芽孢杆菌的粗提物在正常细胞中的存活率为68%，而在癌细胞中为11%（IC为76.9微克/毫升）。根据这些发现，高海拔芽孢杆菌MTCC13046可用于开发有前景的抗癌药物。
