Abdelzaher Walaa Yehia, El-Tahawy Nashwa Fathy Gamal, AboBakr Ali Abdel Hamid Sayed, Mohamed Hatem A, Welson Nermeen N, Aly Labib Dina A
Department of Pharmacology, Faculty of Medicine, Minia University, 61511 Minia, Egypt.
Department of Histology and Cell Biology, Faculty of Medicine, Minia University, 61511 Minia, Egypt.
Int Immunopharmacol. 2023 Jan;114:109492. doi: 10.1016/j.intimp.2022.109492. Epub 2022 Nov 29.
The current experiment aimed to identify the possible protective role of rivastigmine (RIVA) in gentamicin (GNT)-induced acute kidney injury (AKI) in rats. RIVA was administered in the presence and absence of GNT. Kidney function markers and serum and renal GNT concentrations were measured. Renal oxidative stress parameters as well as inflammatory and apoptotic biomarkers were evaluated. Renal histopathological assessment and nuclear factor kappa-B (NF-κB) immunohistochemical study were performed. GNT administration increased serum creatinine, urea, and cystatin C concentrations. RIVA ameliorated these changes via mitigating GNT-induced increases of renal oxidative stress, inflammation, and apoptotic parameters. RIVA showed a prompt improvement in the histopathological renal damage and a decrease in NF-κB immunoexpression. In conclusion, RIVA protective effects against GNT-induced AKI are mediated by decreasing GNT concentration in renal tissue and other effects like antioxidant and antiapoptotic effects possibly through its cholinergic anti-inflammatory action.
当前实验旨在确定卡巴拉汀（RIVA）在庆大霉素（GNT）诱导的大鼠急性肾损伤（AKI）中可能的保护作用。在有和没有GNT的情况下给予RIVA。测量肾功能标志物以及血清和肾脏中的GNT浓度。评估肾脏氧化应激参数以及炎症和凋亡生物标志物。进行肾脏组织病理学评估和核因子κB（NF-κB）免疫组织化学研究。给予GNT会增加血清肌酐、尿素和胱抑素C浓度。RIVA通过减轻GNT诱导的肾脏氧化应激、炎症和凋亡参数的增加来改善这些变化。RIVA显示出肾脏组织病理学损伤迅速改善，NF-κB免疫表达降低。总之，RIVA对GNT诱导的AKI的保护作用是通过降低肾组织中GNT浓度以及其他作用介导的，如抗氧化和抗凋亡作用，可能是通过其胆碱能抗炎作用。
