Meat Processing Key Laboratory of Sichuan Province, School of Food and Biological Engineering, Chengdu University, No. 2025 Chengluo Avenue, Chengdu 610106, China.
Meat Processing Key Laboratory of Sichuan Province, School of Food and Biological Engineering, Chengdu University, No. 2025 Chengluo Avenue, Chengdu 610106, China; Engineering Research Center of Sichuan-Tibet Traditional Medicinal Plant, School of Food and Biological Engineering, Chengdu University, No. 2025 Chengluo Avenue, Chengdu 610106, China.
Food Res Int. 2022 Dec;162(Pt B):112135. doi: 10.1016/j.foodres.2022.112135. Epub 2022 Nov 17.
The physiological status of Salmonella after its ultrasonication was investigated to reveal the potential mechanism through which ultrasound enhances the lethality of chlorine dioxide against Salmonella. Applying either the probe ultrasound (US) or water bath ultrasound (WUS) disrupted the cellular structure of Salmonella bacteria, increased the permeability of their bacterial outer membrane (US: 9.00 %, WUS: 11.96 %), and caused intracellular reactive oxygen species to accumulate (US: 13.95 %, WUS: 4.34 %,), which resulted in a reduction of ATP (US: 15.22 %, WUS: 14.15 %) and ATPase activity (US: 3.13 %, WUS: 26.06 %). This series of adverse effects eventually led to the disruption of the metabolic process in Salmonella cells, by mainly altering the metabolism of lipids, small molecules, and energy. Therefore, ultrasound enhances the lethality of chlorine dioxide primarily by disrupting the cellular structure, intracellular material, and energy homeostasis of Salmonella. This finding will promote the development and application of ultrasonic-assisted sterilization technology in food industries.
研究了超声处理后沙门氏菌的生理状态，以揭示超声增强二氧化氯对沙门氏菌致死率的潜在机制。应用探针超声（US）或水浴超声（WUS）均可破坏沙门氏菌的细胞结构，增加其细菌外膜的通透性（US：9.00%，WUS：11.96%），并导致细胞内活性氧的积累（US：13.95%，WUS：4.34%），这导致 ATP 的减少（US：15.22%，WUS：14.15%）和 ATP 酶活性的降低（US：3.13%，WUS：26.06%）。这一系列的不良反应最终导致沙门氏菌细胞代谢过程的中断，主要是通过改变脂质、小分子和能量的代谢。因此，超声主要通过破坏沙门氏菌的细胞结构、细胞内物质和能量平衡来增强二氧化氯的致死率。这一发现将促进超声辅助杀菌技术在食品工业中的发展和应用。
