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耐冻木蛙全身冷冻过程中 mA 相关蛋白的调控。

The regulation of mA-related proteins during whole-body freezing of the freeze-tolerant wood frog.

机构信息

Department of Biology, Carleton University, Ottawa, ON K1S 5B6, Canada.

出版信息

Biochem Cell Biol. 2023 Feb 1;101(1):77-86. doi: 10.1139/bcb-2022-0164. Epub 2022 Dec 3.

Abstract

(also known as ) is one of the few vertebrates that spend extreme winters showing no physiological signs of life. Up to 70% of the total body water of the wood frog freezes as extracellular ice. Survival in extreme conditions requires regulation at transcriptional and translational levels to activate prosurvival pathways. N6-methyladenosine (mA) methylation is one of the most common RNA modifications, regulating transcript processing and translation by executing important functions that affect regulatory pathways in stress conditions. In the study, regulation of mA-related proteins in the liver of was analyzed during 24 h frozen and 8 h thaw conditions. Decreases in the activity of demethylases of 28.44 ± 0.4% and 24.1 ± 0.9% of control values in frozen and thaw tissues, respectively, were observed. Total protein levels of mA methyltransferase complex components methyltransferase-like 14 and Wilm's tumor associated protein were increased by 1.28-fold and 1.42-fold, respectively, during freezing. Demethylase fat mass and obesity, however, showed a decreasing trend, with a significant decrease in abundance during recovery from frozen conditions. Levels of mRNA degraders YTHDF2 and YTHDC2 also decreased under stress. Overall, increased levels of mA methylation complex components, and suppressed levels of readers/erasers, provide evidence for the potential role of RNA methylation in freezing survival and its regulation in a hypometabolic state.

摘要

(也被称为)是极少数在极寒冬季仍表现出无生命生理迹象的脊椎动物之一。木蛙的总体液多达 70%会形成细胞外冰。在极端条件下生存需要在转录和翻译水平上进行调节,以激活生存途径。N6-甲基腺苷(m6A)甲基化是最常见的 RNA 修饰之一,通过执行影响应激条件下调节途径的重要功能,调节转录本处理和翻译。在这项研究中,分析了 24 小时冷冻和 8 小时解冻条件下木蛙肝脏中 mA 相关蛋白的调节。在冷冻和解冻组织中,去甲基酶的活性分别下降了 28.44±0.4%和 24.1±0.9%。在冷冻过程中,m6A 甲基转移酶复合物成分甲基转移酶样蛋白 14 和 Wilm 瘤相关蛋白的总蛋白水平分别增加了 1.28 倍和 1.42 倍。然而,去甲基酶脂肪量和肥胖症表现出下降趋势,在从冷冻条件中恢复时丰度显著下降。mRNA 降解酶 YTHDF2 和 YTHDC2 的水平在应激下也降低。总的来说,m6A 甲基化复合物成分水平升高,而读码器/擦除器水平降低,为 RNA 甲基化在冷冻生存中的潜在作用及其在低代谢状态下的调节提供了证据。

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