紫茎泽兰精油化学型对捻转血矛线虫的体外驱虫活性。
In vitro anthelmintic activity of Lippia alba essential oil chemotypes against Haemonchus contortus.
机构信息
Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Brazil.
Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Brazil.
出版信息
Exp Parasitol. 2023 Jan;244:108439. doi: 10.1016/j.exppara.2022.108439. Epub 2022 Dec 1.
The aim of the present study was to evaluate the effects of the essential oils of Lippia alba chemotypes carvone and citral on H. contortus. Chemical characterization was performed by means of gas chromatography coupled to mass spectrometry (GC/MS). The anthelmintic effects of the essential oils were assessed through the egg hatch test (EHT) and the adult worm motility test (AWMT) using a multidrug-resistant H. contortus Kokstad isolate. Confocal laser scanning microscopy (CLSM) of eggs and adults of H. contortus and scanning electron microscopy (SEM) of adults were performed after treatment with oils for qualitative observations of their effects. The carvone chemotype of L. alba (LaCV) presented 70% carvone, and the citral chemotype of L. alba (LaCT) presented 29.4% geranial and 20.4% neral, respectively. In the EHT, the EC50 values of LaCV and LaCT were 0.2 and 0.3 mg/mL, respectively. In AWMT, after 12 h of exposure to 2 mg/mL LaCV and 2 mg/mL LaCT, 100% of adult nematodes were immobile. CLSM showed changes in larval motility inside the egg caused by LaCV, while LaCT promoted changes in larval formation. In adults exposed to both chemotypes, alterations in the anterior portion of the oesophagus were observed. In SEM, morphological changes were observed in the buccal capsule and in the medial portion of H. contortus adults. It is concluded that the two essential oils of L. alba, the chemotypes carvone and citral, caused morphological changes and inhibited the hatching of eggs and the motility of adult H. contortus nematodes.
本研究旨在评估 Lippia alba 化学型香芹酮和柠檬醛精油对旋毛虫的影响。采用气相色谱-质谱联用（GC/MS）对精油进行化学特征分析。通过卵孵化试验（EHT）和多药耐药旋毛虫 Kokstad 分离株的成虫运动试验（AWMT）评估精油的驱虫效果。用精油处理旋毛虫的卵和成虫后，进行共聚焦激光扫描显微镜（CLSM）和扫描电子显微镜（SEM）观察，定性观察其作用。白千层的香芹酮化学型（LaCV）含有 70%的香芹酮，而白千层的柠檬醛化学型（LaCT）分别含有 29.4%的牻牛儿醛和 20.4%的橙花醛。在 EHT 中，LaCV 和 LaCT 的 EC50 值分别为 0.2 和 0.3 mg/mL。在 AWMT 中，暴露于 2 mg/mL LaCV 和 2 mg/mL LaCT 12 小时后，100%的成年线虫无运动能力。CLSM 显示 LaCV 引起卵内幼虫运动的变化，而 LaCT 促进幼虫形成的变化。在接触两种化学型的成虫中，观察到食道前部的变化。在 SEM 中，观察到旋毛虫的颊囊和中体部分的形态变化。综上所述，白千层的两种精油，即香芹酮和柠檬醛化学型，引起形态变化并抑制旋毛虫卵的孵化和成虫的运动。
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