College of Urban and Environmental Sciences, Peking University, Beijing, 100871, People's Republic of China.
College of Environment and Safety Engineering, Fujian Province, Fuzhou University, Fuzhou University Town, No. 2 Wulongjiang North Avenue, Fuzhou City, People's Republic of China.
Environ Sci Pollut Res Int. 2023 Mar;30(12):32573-32584. doi: 10.1007/s11356-022-24229-5. Epub 2022 Dec 5.
Karst tiankeng are important biodiversity conservation reservoirs. However, the unique habitats of karst tiankeng affect microbial community structure remained poorly understood. In this study, we collected soil samples from karst tiankeng (TK) and karst land (KL) and subjected to high-throughput sequencing. Based on the classification of the total, abundance, and rare taxa for bacteria and fungi, a multivariate statistical analysis was carried out. The results revealed that bacterial community Shannon diversity and Pielou's evenness were highest in TK. The rare taxa were ubiquitous in all soil samples, while the higher Shannon diversity of the abundant taxa of TK may be related to the habitat preferences of species and niche differentiation. The community composition of bacterial and fungal sub-communities exhibited significant dissimilarity between TK and KL. The redundancy analysis further demonstrated that abundant taxa were environmentally more constrained than rare taxa. The bacterial and fungal networks of KL were more complex than TK. The keystones of the network transforms may suggest their significant role in the ecological function of the karst tiankeng ecosystem. This study represents the first reports of the characteristics of bacterial and fungal communities in karst tiankeng.
喀斯特天坑是重要的生物多样性保护库。然而，喀斯特天坑独特的生境仍不清楚如何影响微生物群落结构。在这项研究中，我们从喀斯特天坑（TK）和喀斯特土地（KL）收集了土壤样本，并进行了高通量测序。基于细菌和真菌的总分类、丰度和稀有分类的分类，进行了多变量统计分析。结果表明，细菌群落 Shannon 多样性和 Pielou 的均匀度在 TK 中最高。稀有分类在所有土壤样本中都普遍存在，而 TK 中丰富分类的更高 Shannon 多样性可能与物种的栖息地偏好和生态位分化有关。细菌和真菌亚群落的群落组成在 TK 和 KL 之间表现出显著的差异。冗余分析进一步表明，丰富分类比稀有分类更受环境的限制。KL 的细菌和真菌网络比 TK 更复杂。网络转换的关键因素可能表明它们在喀斯特天坑生态系统的生态功能中具有重要作用。本研究首次报道了喀斯特天坑中细菌和真菌群落的特征。
