Vitalant Research Institute, 360 Spear Street, Suite 200, 94105, San Francisco, CA, USA.
Department of Laboratory Medicine, University of California, 94141, San Francisco, CA, USA.
BMC Res Notes. 2022 Dec 6;15(1):358. doi: 10.1186/s13104-022-06229-3.
Cell-surface antigen screening was performed on human fetal liver cells using flow cytometry. The goal was to provide proteomic expression data on a number of human fetal liver cell populations that can inform studies on developmental hepatology and hematology.
A 21 weeks' gestation liver was depleted of erythrocytes prior to antibody staining. Screening was performed using phycoerythrin-labelled antibodies against 332 antigens. In addition to these antibodies, all samples were stained for CD14, CD45, CD235a, and CD326 (epithelial cell adhesion molecule - EpCAM). Subpopulations of fetal liver cells were identified using the co-stained antigens. Hematopoietic cells were identified by their expression of CD45 and CD235a; non-hematopoietic cells were further subdivided based on CD14 and CD326 expression. CD326CD14 hepatoblasts and CD14 liver sinusoidal endothelial cells were analyzed for the frequency and intensity of antigen expression. Analyzed flow cytometry data are presented for the expression of the antigens on hematopoietic cells and on non-hematopoietic cells in the context of CD14 and CD326 expression.
通过流式细胞术对人胎肝细胞进行细胞表面抗原筛选。目的是提供大量人胎肝细胞群体的蛋白质组表达数据，为发育性肝和血液学研究提供信息。
在进行抗体染色之前，先从 21 周妊娠的肝脏中去除红细胞。使用针对 332 种抗原的藻红蛋白标记抗体进行筛选。除了这些抗体外，所有样本均用 CD14、CD45、CD235a 和 CD326（上皮细胞黏附分子 - EpCAM）进行染色。使用共同染色的抗原来鉴定胎肝细胞的亚群。通过表达 CD45 和 CD235a 来鉴定造血细胞；非造血细胞进一步根据 CD14 和 CD326 的表达进行细分。分析了 CD326CD14 肝母细胞和 CD14 肝窦内皮细胞的抗原表达频率和强度。分析的流式细胞术数据显示了在 CD14 和 CD326 表达的背景下，造血细胞和非造血细胞上抗原的表达情况。
