Hou Anwei, Dickschat Jeroen S
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, West 7th Avenue No. 32, 300308 Tianjin, China.
Institute of Microbiology, Jiangxi Academy of Sciences, Changdong Road No. 7777, 330096 Nanchang, China.
Nat Prod Rep. 2023 Feb 22;40(2):470-499. doi: 10.1039/d2np00071g.
Covering: 2015 to 2022In this review, we discuss the recent advances in the use of isotopically labelled compounds to investigate the biosynthesis of polyketides, non-ribosomally synthesised peptides, and their hybrids. Also, we highlight the use of isotopes in the elucidation of their structures and investigation of enzyme mechanisms. The biosynthetic pathways of selected examples are presented in detail to reveal the principles of the discussed labelling experiments. The presented examples demonstrate that the application of isotopically labelled compounds is still the state of the art and can provide valuable information for the biosynthesis of natural products.
2015年至2022年
在本综述中，我们讨论了使用同位素标记化合物研究聚酮化合物、非核糖体合成肽及其杂合物生物合成的最新进展。此外，我们强调了同位素在阐明其结构和研究酶机制方面的应用。详细介绍了选定实例的生物合成途径，以揭示所讨论的标记实验的原理。所举实例表明，同位素标记化合物的应用仍然是最先进的技术，可为天然产物的生物合成提供有价值的信息。
