人类诱导多能干细胞衍生的视网膜类器官发育的批量 RNA 测序分析。
Bulk RNA sequencing analysis of developing human induced pluripotent cell-derived retinal organoids.
机构信息
Viterbi Family Department of Ophthalmology at the Shiley Eye Institute, University of California San Diego, La Jolla, CA, 92093, USA.
Department of Bioengineering, University of California San Diego, La Jolla, CA, 92093, USA.
出版信息
Sci Data. 2022 Dec 9;9(1):759. doi: 10.1038/s41597-022-01853-x.
Retinogenesis involves the transformation of the anterior developing brain into organized retinal lamellae coordinated by intricate gene signalling networks. This complex process has been investigated in several model organisms such as birds, fish, mammals and amphibians, yet many facets of retinal development are different in humans and remain unexplored. In this regard, human pluripotent stem cell (hPSC)-derived 3D retinal organoids and Next Generation Sequencing (NGS) have emerged as key technologies that have facilitated the discovery of previously unknown details about cell fate specification and gene regulation in the retina. Here we utilized hPSCs integrated with fluorescent reporter genes (SIX6-p2A-eGFP/CRX-p2A-h2b-mRuby3) to generate retinal organoids and carry out bulk RNA sequencing of samples encompassing the majority of retinogenesis (D0-D280). This data set will serve as a valuable reference for the vision research community to characterize differentially expressed genes in the developing human eye.
视网膜发生涉及到由复杂的基因信号网络协调的前脑发育成有组织的视网膜层。这个复杂的过程已经在鸟类、鱼类、哺乳动物和两栖动物等几种模式生物中进行了研究，但人类的视网膜发育有许多方面是不同的，仍有待探索。在这方面，人类多能干细胞（hPSC）衍生的 3D 视网膜类器官和下一代测序（NGS）已经成为关键技术，它们促进了对视网膜细胞命运特化和基因调控的先前未知细节的发现。在这里，我们利用整合了荧光报告基因（SIX6-p2A-eGFP/CRX-p2A-h2b-mRuby3）的 hPSC 来生成视网膜类器官，并对包括大多数视网膜发生（D0-D280）在内的样本进行批量 RNA 测序。这个数据集将成为视觉研究社区的宝贵参考，用于描述人眼发育过程中差异表达的基因。
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