黄花蒿对淡色库蚊的杀幼虫和驱避活性。
Larvicidal and repellent potential of Ageratum houstonianum against Culex pipiens.
机构信息
Department of Medicinal Plants and Natural Products, National Organization for Drug Control and Research (NODCAR), 51-Wezaret El-Zeraa St, Giza, 12611, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr El Aini, 11562, Egypt.
出版信息
Sci Rep. 2022 Dec 10;12(1):21410. doi: 10.1038/s41598-022-25939-z.
Mosquitoes are unquestionably the most medic arthropod vectors of disease. Culex pipiens, usually defined as a common house mosquito, is a well-known carrier of several virus diseases. Crude ethanol extracts of different organs of Agratum houstonianum are tested with Culex pipiens Linnaeus (Diptera: Culicidae) to determine their larvicidal, antifeedant, and repellency effects. Alongside biochemical analysis, the activity of the AChE, ATPase, CarE, and CYP-450 is detected in the total hemolymph of the C. pipiens larvae to examine the enzymatic action on the way to explain their neurotoxic effect and mode of action. Through HPLC and GC-MS analysis of the phytochemical profile of A. houstonianum aerial parts is identified. The larvicidal activity of aerial parts; flower (AF), leaf (AL), and stem (AS) of A. houstonianum extracts are evaluated against the 3rd instar larvae of C. pipiens at 24-, 48- and 72-post-treatment. A. houstonianium AF, AL, and AS extracts influenced the mortality of larvae with LC50 values 259.79, 266.85, and 306.86 ppm, respectively after 24 h of application. The potency of AF and AL extracts was 1.69- and 1.25-folds than that of AS extract, respectively. A high repellency percentage was obtained by AF extract 89.10% at a dose of 3.60 mg/cm. A. houstonianium AF prevailed inhibition on acetylcholinesterase and decrease in carboxylesterase activity. Moreover, a significant increase in the ATPase levels and a decrease in cytochrome P-450 monooxegenase activity (- 36.60%) are detected. HPLC analysis prevailed chlorogenic and rosmarinic acid as the major phenolic acids in AL and AF, respectively. GC-MS analysis of A. houstonianum results in the identification of phytol as the major makeup. Precocene I and II were detected in AF. Linoleic, linolenic, and oleic acid were detected in comparable amounts in the studied organs. Overall, results suggest that the A. houstonianum flower extract (AF) exhibits significant repellent, antifeedant, and larvicidal activities.
毫无疑问，蚊子是最具医学意义的节肢动物病媒。库蚊通常被定义为常见的家蚊，是几种病毒病的已知载体。用库蚊幼虫（双翅目：蚊科）测试不同器官的粗乙醇提取物，以确定其杀幼虫、拒食和驱避作用。除了生化分析外，还检测了库蚊幼虫总血淋巴中的 AChE、ATPase、CarE 和 CYP-450 的活性，以检测其对神经毒性作用和作用方式的酶促作用。通过对空中部分的植物化学图谱进行 HPLC 和 GC-MS 分析，鉴定了其中的化学成分。评估了空中部分；花（AF）、叶（AL）和茎（AS）的杀幼虫活性对 3 龄幼虫的影响库蚊幼虫在 24、48 和 72 后处理小时。24 小时后，AF、AL 和 AS 提取物对幼虫的致死率分别为 LC50 值 259.79、266.85 和 306.86 ppm。AF 和 AL 提取物的效力分别是 AS 提取物的 1.69-和 1.25-倍。AF 提取物在 3.60 mg/cm 剂量下获得 89.10%的高驱避率。AF 提取物对乙酰胆碱酯酶有强烈的抑制作用，羧基酯酶活性降低。此外，还检测到 ATPase 水平显著增加和细胞色素 P-450 单加氧酶活性降低（-36.60%）。HPLC 分析显示，AL 和 AF 中的主要酚酸分别为绿原酸和迷迭香酸。GC-MS 分析结果表明，植醇是主要成分。AF 中检测到 Precocene I 和 II。在研究的器官中，检测到亚油酸、亚麻酸和油酸的含量相当。总的来说，结果表明，A. houstonianum 花提取物（AF）表现出显著的驱避、拒食和杀幼虫活性。
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