Laboratory of Experimental and Clinical Neurosciences, INSERM U-1084, University of Poitiers, CEDEX 09, 86073 Poitiers, France.
Cells. 2022 Nov 29;11(23):3831. doi: 10.3390/cells11233831.
Traumatic brain injury (TBI) causes cell death mainly in the cerebral cortex. We have previously reported that transplantation of embryonic cortical neurons immediately after cortical injury allows the anatomical reconstruction of injured pathways and that a delay between cortical injury and cell transplantation can partially improve transplantation efficiency. Biomaterials supporting repair processes in combination with cell transplantation are in development. Hyaluronic acid (HA) hydrogel has attracted increasing interest in the field of tissue engineering due to its attractive biological properties. However, before combining the cell with the HA hydrogel for transplantation, it is important to know the effects of the implanted hydrogel alone. Here, we investigated the therapeutic effect of HA on host tissue after a cortical trauma. For this, we implanted HA hydrogel into the lesioned motor cortex of adult mice immediately or one week after a lesion. Our results show the vascularization of the implanted hydrogel. At one month after HA implantation, we observed a reduction in the glial scar around the lesion and the presence of the newly generated oligodendrocytes, immature and mature neurons within the hydrogel. Implanted hydrogel provides favorable environments for the survival and maturation of the newly generated neurons. Collectively, these results suggest a beneficial effect of biomaterial after a cortical traumatic injury.
创伤性脑损伤 (TBI) 主要导致大脑皮层中的细胞死亡。我们之前曾报道过，在皮质损伤后立即移植胚胎皮质神经元可以实现损伤通路的解剖重建，并且在皮质损伤和细胞移植之间存在时间延迟可以部分提高移植效率。支持修复过程的生物材料与细胞移植相结合正在开发中。由于具有吸引人的生物学特性，透明质酸 (HA) 水凝胶在组织工程领域引起了越来越多的关注。然而，在将细胞与 HA 水凝胶结合用于移植之前，了解单独植入的水凝胶的影响很重要。在这里，我们研究了 HA 在皮质损伤后对宿主组织的治疗作用。为此，我们将 HA 水凝胶立即或损伤后一周植入成年小鼠的损伤运动皮层。我们的结果显示了植入水凝胶的血管化。在 HA 植入后一个月，我们观察到损伤周围的神经胶质瘢痕减少，并且在水凝胶内存在新生成的少突胶质细胞、未成熟和成熟神经元。植入的水凝胶为新生成的神经元的存活和成熟提供了有利的环境。总之，这些结果表明生物材料在皮质创伤后具有有益的作用。
