Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China.
State Key Laboratory Breeding Base of Green Pesticide & Agricultural Bioengineering, Key Laboratory of Green Pesticide & Agricultural Bioengineering, Ministry of Education, State-Local Joint Laboratory for Comprehensive Utilization of Biomass, Center for R&D of Fine Chemicals, Guizhou University, Guiyang 550025, China.
Molecules. 2022 Dec 5;27(23):8559. doi: 10.3390/molecules27238559.
Since antimicrobials were banned as feed additives, coccidiostats with favorable anticoccidial action and growth promotion have been widely used in the breeding industry. The monitoring of coccidiostats in feed is necessary, while the current methods based on mass-spectrometer analysis have limited applicability and matrix effects could interfere with the results. Accordingly, in the present paper, a rapid analytical strategy for the simultaneous determination of six synthetic coccidiostats in feed using high-performance liquid chromatography coupled with diode-array detection was developed. Coccidiostats in chicken feeds were extracted with the trichloroacetic acid-acetonitrile solution. The cleanup was performed by dispersive solid-phase extraction after the optimization of the response surface methodology. The method exhibited good linearity for target coccidiostats within the range of 0.0520 µg/mL. Recoveries for six compounds in fortified feed samples were from 67.2% to 107.2% with relative standard deviations less than 9.6%. The limit of detection was 0.20.3 mg/kg. The successful application of the method in commercial feed verified that it is effective and sensitive for the rapid determination of multiple coccidiostats in chicken feeds.
自抗生素被禁止作为饲料添加剂以来，具有良好抗球虫作用和促生长作用的球虫抑制剂已被广泛应用于养殖业。对饲料中的球虫抑制剂进行监测是必要的，而目前基于质谱分析的方法适用性有限，基质效应可能会干扰结果。因此，本研究建立了一种利用高效液相色谱-二极管阵列检测法同时测定饲料中 6 种合成球虫抑制剂的快速分析策略。用三氯乙酸-乙腈溶液提取鸡饲料中的球虫抑制剂。通过响应面法优化后，采用分散固相萃取进行净化。该方法在 0.0520 µg/mL 范围内对目标球虫抑制剂表现出良好的线性关系。6 种化合物在添加饲料样品中的回收率为 67.2%107.2%，相对标准偏差小于 9.6%。检出限为 0.2~0.3 mg/kg。该方法在商业饲料中的成功应用验证了它可有效、灵敏地快速测定鸡饲料中的多种球虫抑制剂。
