Martínez-Solís Jesús, Calzada Fernando, Barbosa Elizabeth, Gutiérrez-Meza Juan Manuel
Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina (ESM), Instituto Politécnico Nacional, Plan de San Luis y Salvador Díaz Mirón S/N, Col. Casco de Santo Tomás, Mexico City CP 11340, Mexico.
Unidad de Investigación Médica en Farmacología, UMAE Hospital de Especialidades 2° Piso CORSE Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av. Cuauhtémoc 330, Col. Doctores, Mexico City CP 06720, Mexico.
Plants (Basel). 2022 Nov 24;11(23):3224. doi: 10.3390/plants11233224.
Miller () is a plant used in Mexican traditional medicine for the treatment of diabetes. In this work, the tea infusion extracts obtained from 1.5 g of leaf powder from collected in May (AcMa), June (AcJun), July (AcJul), and August (AcAu) were evaluated on streptozocin-induced diabetic (STID) mice and for subchronic toxicity in STID and non-diabetic (ND) mice. In addition, extracts were subjected to high-performance liquid chromatography with diode array detection (HPLC-DAD). Results showed that the tea infusion extract of the sample collected in August (AcAu) exhibited the most significant antihyperglycemic activity during all acute assays. The analysis of the extracts (AcMa, AcJu, AcJul, and AcAu) by HPLC-DAD revealed that flavonoid glycosides, rutin, narcissin, and nicotiflorin were the major components. In addition, the sample AcAu contained the best concentration of flavonoids. In the case of subchronic oral toxicity, the AcAu sample did not cause mortality in STID mice, and histopathological analysis revealed significant improvement in the changes associated with diabetes in the liver and kidneys. These findings suggest that the leaves collected in August may be a source of flavonoids such as rutin, with antidiabetic potential. In addition, these findings support the use of to treat diabetes in traditional medicine.
米勒（）是一种在墨西哥传统医学中用于治疗糖尿病的植物。在这项研究中，对从5月（AcMa）、6月（AcJun）、7月（AcJul）和8月（AcAu）采集的1.5克叶粉获得的茶浸提取物在链脲佐菌素诱导的糖尿病（STID）小鼠上进行了评估，并对STID和非糖尿病（ND）小鼠进行了亚慢性毒性评估。此外，提取物还进行了带二极管阵列检测的高效液相色谱分析（HPLC-DAD）。结果表明，8月采集的样品（AcAu）的茶浸提取物在所有急性试验中表现出最显著的降血糖活性。通过HPLC-DAD对提取物（AcMa、AcJu、AcJul和AcAu）的分析表明，黄酮苷、芦丁、水仙苷和烟花苷是主要成分。此外，AcAu样品中黄酮类化合物的浓度最高。在亚慢性口服毒性方面，AcAu样品在STID小鼠中未导致死亡，组织病理学分析显示肝脏和肾脏中与糖尿病相关的变化有显著改善。这些发现表明，8月采集的叶子可能是芦丁等具有抗糖尿病潜力的黄酮类化合物的来源。此外，这些发现支持了在传统医学中使用治疗糖尿病。
