Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Allergy and Clinical Immunology, Pediatric Hospital, Tabriz University of Medical Sciences, Tabriz, Iran.
BMC Res Notes. 2022 Dec 12;15(1):366. doi: 10.1186/s13104-022-06253-3.
From the ancient, medicinal benefits of Hyssop (Hyssopus officinalis L.) have been implicated for respiratory and digestive diseases despite the effects of Hyssop on viral infections have not been mechanistically investigated. In this study, we examined whether the Hyssop extract activated anti-viral innate immunity, as a sentinel for immune system, through activation of endosomal TLRs recognizing nucleic acids and their downstream signaling. The Hyssop herb extracts was prepared and co-cultured with healthy individual's peripheral blood mononuclear cells (PBMCs). After viability assay, gene expression levels of TLR3,7,8,9, as well as MyD88 and NF-κB, were evaluated in treated PBMCs using Real-time PCR. Next, the secretion level of immune related cytokines was quantified via ELISA.
Post 24 h, 40 µg/ml of the extract significantly inhibited the viability of less than 50% of cells compared to the control and had a maximum effect on cellular function. The Hyssop-treated PBMCs demonstrated an elevated expression of endosomal TLRs genes, as well as MyD88 and NF-κB. Moreover, the release of INF-α and β notably enhanced in cell culture supernatant, while the content of inflammatory cytokines remarkably diminished (P < 0.05). The Hyssop extract was capable of inducing antiviral innate immune responses so can be promising in antiviral drug strategies.
尽管尚未对其抗病毒感染的机制进行研究，但自古以来，药用甘菊（Hyssopus officinalis L.）就被认为对呼吸和消化系统疾病有疗效。在这项研究中，我们通过激活识别核酸的内体 TLR 及其下游信号转导，研究了甘菊提取物是否通过激活抗病毒先天免疫作为免疫系统的哨兵来发挥作用。我们制备了甘菊草药提取物并与健康个体的外周血单核细胞（PBMC）共培养。通过活力测定后，使用实时 PCR 评估了 TLR3、7、8、9 以及 MyD88 和 NF-κB 的基因表达水平。然后，通过 ELISA 定量测定免疫相关细胞因子的分泌水平。
与对照组相比，提取后 24 小时，40μg/ml 的提取物对细胞活力的抑制作用小于 50%，对细胞功能的影响最大。甘菊处理的 PBMC 表现出内体 TLR 基因、MyD88 和 NF-κB 的表达水平升高。此外，细胞培养上清液中 INF-α和β的释放明显增加，而炎性细胞因子的含量明显减少（P<0.05）。甘菊提取物能够诱导抗病毒先天免疫反应，因此在抗病毒药物策略中具有广阔的前景。
