School of Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, China.
Pukou Agricultural Technology Extension Center of Nanjing City, Pukou, China.
Bull Entomol Res. 2023 Apr;113(2):282-291. doi: 10.1017/S0007485322000578. Epub 2022 Dec 12.
is a significant pest of vegetable and ornamental crops across the globe. Microwave radiation has been used for controlling pests in stored products; however, there are few reports on the use of microwaves for eradicating agricultural pests such as , and its effects on pests at the molecular level is unclear. In this study, we show that microwave radiation inhibited the emergence of pupae. Transcriptomic studies of indicated significant enrichment of differentially expressed genes (DEGs) in 'post-translational modification, protein turnover, chaperones', 'sensory perception of pain/transcription repressor complex/zinc ion binding' and 'insulin signaling pathway' when analyzed with the Clusters of Orthologous Groups, Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes databases, respectively. The top DEGs were related to reproduction, immunity and development and were significantly expressed after microwave radiation. Interestingly, there was no significant difference in the expression of genes encoding heat shock proteins or antioxidant enzymes in treated with microwave radiation as compared to the untreated control. The expression of DEGs encoding cuticular protein and protein takeout were silenced by RNA interference, and the results showed that knockdown of these two DEGs reduced the survival of exposed to microwave radiation. The results of this study help elucidate the molecular response of exposed to microwave radiation and provide novel ideas for control.
小菜蛾是一种全球性的蔬菜和观赏作物的重要害虫。微波辐射已被用于控制储存产品中的害虫；然而，关于微波用于根除农业害虫如小菜蛾的报道很少，其对害虫的分子水平的影响尚不清楚。在本研究中，我们表明微波辐射抑制了小菜蛾蛹的羽化。通过使用同源基因簇、基因本体论和京都基因与基因组百科全书数据库分别对 进行分析，发现转录组研究表明，在“翻译后修饰、蛋白质周转、伴侣”、“疼痛/转录阻遏复合物/锌离子结合的感觉感知”和“胰岛素信号通路”中差异表达基因（DEGs）显著富集。顶级 DEGs 与生殖、免疫和发育有关，在微波辐射后表达显著。有趣的是，与未经微波辐射处理的对照组相比，经微波辐射处理的 中编码热休克蛋白或抗氧化酶的基因表达没有显著差异。通过 RNA 干扰沉默编码表皮蛋白和蛋白提取的 DEGs 的表达，结果表明这两个 DEGs 的敲低降低了暴露于微波辐射的 的存活率。本研究的结果有助于阐明 暴露于微波辐射的分子反应，并为控制提供新的思路。
