College of Plant Protection, Yangzhou University, Yangzhou, China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education, Yangzhou University, Yangzhou, China.
Bull Entomol Res. 2024 Apr;114(2):230-236. doi: 10.1017/S0007485324000026. Epub 2024 Mar 13.
As an environmental factor, temperature impacts the distribution of species and influences interspecific competition. The molecular chaperones encoded by small heat shock proteins (sHsps) are essential for rapid, appropriate responses to environmental stress. This study focuses on , which encodes a temperature-responsive in , an insect pest that infests both agricultural and ornamental crops. expression was highest at 39℃ in pupae and adults, and expression levels were greater in pupae than in adults. Recombinant was expressed in and conferred a higher survival rate than the empty vector to bacterial cells exposed to heat stress. RNA interference experiments were conducted using adults and prepupae and the knockdown of expression increased mortality in during heat stress. The results expand our understanding of function in spp. and the ongoing adaptation of this pest to climate change. In addition, this study is also important for predicting the distribution of invasive species and proposing new prevention and control strategies based on temperature adaptation.
作为环境因素之一，温度会影响物种的分布，并影响种间竞争。小分子热休克蛋白（sHsps）编码的分子伴侣对于快速、适当的环境应激反应至关重要。本研究聚焦于 ，它编码一种温度响应的 在农业和观赏作物害虫中普遍存在。 在蛹和成虫中的表达量在 39℃时最高，且蛹中的表达水平高于成虫。在 中表达重组 ，使其在暴露于热应激的细菌细胞中比空载体具有更高的存活率。使用 成虫和预蛹进行 RNA 干扰实验，敲低 表达会增加 热应激期间的死亡率。这些结果扩展了我们对 功能的理解，并为这种害虫对气候变化的持续适应提供了新的认识。此外，本研究对于预测入侵物种的分布以及提出基于温度适应的新的预防和控制策略也具有重要意义。
