三阴性乳腺癌中环状RNA的预测与筛选
Prediction and screening of circRNA in triple-negative breast cancer.
作者信息
Li Xuanhe, Zhang Song, Sun Siyu, Yue Xicheng, Qian Liyu, Tang Jie, Jiang Fangqian, Lu Jianfei, Cao Yifan, Meng Shengwen, Yao Tingjing
机构信息
Oncology Surgery, The First Affiliated Hospital of Bengbu Medical College 287 Changhuai Road, Bengbu 233000, Anhui, China.
出版信息
Am J Transl Res. 2022 Nov 15;14(11):8049-8063. eCollection 2022.
OBJECTIVE
The purpose of this investigation was to study the expression profile and potential function of circular RNA (circRNA) and long noncoding RNA (lncRNA) in triple-negative breast cancer (TNBC).
METHODS
RNA sequencing technology was used to detect differentially expressed circRNAs and lncRNAs between TNBC tissues and the adjacent tissue. The potential functions of these different RNAs were analyzed by GO and KEGG enrichment analysis by bioinformatics tools. We also selected and analyzed these key circRNAs and lncRNAs to verify their important functions in TNBC.
RESULTS
A total of 139 differentially expressed circRNAs and 1001 lncRNAs were obtained. The co-expression analysis showed that the hub lncRNAs (OIP5-AS1, DRAIC) were associated with several tumors and mainly enriched in tumor metastasis. We also screened 5 circRNA-hosting genes (NTRK2, FNTA, BAPGEF2, MGST2, ADH1B) that were associated with the brain-derived neurotrophic factor (BDNF) receptor signaling pathway and cerebral cortex development, as well as AMPK and TGF-β signaling pathway.
CONCLUSION
We identified a large number of differentially expressed circRNAs and lncRNAs, which provide useful insight in understanding TNBC carcinogenesis.
目的
本研究旨在探讨环状RNA（circRNA）和长链非编码RNA（lncRNA）在三阴性乳腺癌（TNBC）中的表达谱及潜在功能。
方法
采用RNA测序技术检测TNBC组织与癌旁组织中差异表达的circRNA和lncRNA。利用生物信息学工具通过GO和KEGG富集分析对这些差异RNA的潜在功能进行分析。我们还筛选并分析了这些关键的circRNA和lncRNA，以验证它们在TNBC中的重要功能。
结果
共获得139个差异表达的circRNA和1001个lncRNA。共表达分析显示，枢纽lncRNA（OIP5-AS1、DRAIC）与多种肿瘤相关，主要富集于肿瘤转移。我们还筛选出5个circRNA宿主基因（NTRK2、FNTA、BAPGEF2、MGST2、ADH1B），它们与脑源性神经营养因子（BDNF）受体信号通路、大脑皮层发育以及AMPK和TGF-β信号通路相关。
结论
我们鉴定出大量差异表达的circRNA和lncRNA，这为理解TNBC的致癌机制提供了有益的见解。
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