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大鼠脊髓背角中内吗啡肽-2与脊髓臂旁投射神经元的形态学研究

Morphological investigations of endomorphin-2 and spinoparabrachial projection neurons in the spinal dorsal horn of the rat.

作者信息

Yin Jun-Bin, Lu Ya-Cheng, Li Fei, Zhang Ting, Ding Tan, Hu Huai-Qiang, Chen Ying-Biao, Guo Hong-Wei, Kou Zhen-Zhen, Zhang Ming-Ming, Yuan Jun, Chen Tao, Li Hui, Cao Bing-Zhen, Dong Yu-Lin, Li Yun-Qing

机构信息

Department of Human Anatomy, K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an, China.

Department of Neurology, The 960th Hospital of Joint Logistics Support, PLA, Jinan, China.

出版信息

Front Neuroanat. 2022 Nov 23;16:1072704. doi: 10.3389/fnana.2022.1072704. eCollection 2022.

DOI:10.3389/fnana.2022.1072704
PMID:36506871
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9726772/
Abstract

It has been proved that endomorphin-2 (EM2) produced obvious analgesic effects in the spinal dorsal horn (SDH), which existed in our human bodies with remarkable affinity and selectivity for the μ-opioid receptor (MOR). Our previous study has demonstrated that EM2 made synapses with the spinoparabrachial projection neurons (PNs) in the SDH and inhibited their activities by reducing presynaptic glutamate release. However, the morphological features of EM2 and the spinoparabrachial PNs in the SDH have not been completely investigated. Here, we examined the morphological features of EM2 and the spinoparabrachial PNs by using triple fluorescence and electron microscopic immunohistochemistry. EM2-immunoreactive (-ir) afferents directly contacted with the spinoparabrachial PNs in lamina I of the SDH. Immunoelectron microscopy (IEM) were used to confirm that these contacts were synaptic connections. It was also observed that EM2-ir axon terminals contacting with spinoparabrachial PNs in lamina I contained MOR, substance P (SP) and vesicular glutamate transporter 2 (VGLUT2). In lamina II, MOR-ir neurons were observed to receive direct contacts from EM2-ir varicosities. The synaptic connections among EM2, MOR, SP, VGLUT2, and the spinoparabrachial PNs were also confirmed by IEM. In sum, our results supply morphological evidences for the analgesic effects of EM2 on the spinoparabrachial PNs in the SDH.

摘要

已证实内吗啡肽 -2（EM2）在脊髓背角（SDH）产生明显的镇痛作用，其在人体中存在，对μ - 阿片受体（MOR）具有显著的亲和力和选择性。我们之前的研究表明，EM2与SDH中的脊髓臂旁投射神经元（PNs）形成突触，并通过减少突触前谷氨酸释放来抑制其活动。然而，SDH中EM2和脊髓臂旁PNs的形态学特征尚未得到充分研究。在此，我们使用三重荧光和电子显微镜免疫组织化学检查了EM2和脊髓臂旁PNs的形态学特征。EM2免疫反应性（-ir）传入纤维直接与SDH第I层中的脊髓臂旁PNs接触。免疫电子显微镜（IEM）用于证实这些接触是突触连接。还观察到，在第I层中与脊髓臂旁PNs接触的EM2 - ir轴突终末含有MOR、P物质（SP）和囊泡谷氨酸转运体2（VGLUT2）。在第II层中，观察到MOR - ir神经元接受来自EM2 - ir曲张体的直接接触。IEM也证实了EM2、MOR、SP、VGLUT2和脊髓臂旁PNs之间的突触连接。总之，我们的结果为EM2对SDH中脊髓臂旁PNs的镇痛作用提供了形态学证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/64d814950b8a/fnana-16-1072704-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/3bca3cfe7ba0/fnana-16-1072704-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/91854e0003dc/fnana-16-1072704-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/f6743bb0a3c0/fnana-16-1072704-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/65de239ef14b/fnana-16-1072704-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/38c505544cf6/fnana-16-1072704-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/fc0f3b823f63/fnana-16-1072704-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/1694f8b079cc/fnana-16-1072704-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/64d814950b8a/fnana-16-1072704-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/3bca3cfe7ba0/fnana-16-1072704-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/91854e0003dc/fnana-16-1072704-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/f6743bb0a3c0/fnana-16-1072704-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/65de239ef14b/fnana-16-1072704-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/38c505544cf6/fnana-16-1072704-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/fc0f3b823f63/fnana-16-1072704-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/1694f8b079cc/fnana-16-1072704-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88b7/9726772/64d814950b8a/fnana-16-1072704-g008.jpg

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