Kiani Marjan, Zabihi Ebrahim, Nafarzadeh Shima, Nouri Hamid Reza, Bijani Ali, Seyedmajidi Maryam
Student Research Committee, Postgraduate Student Dept. of Oral and Maxillofacial Pathology, Babol University of Medical Sciences, Babol, Iran.
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
J Dent (Shiraz). 2022 Sep;23(3):257-265. doi: 10.30476/DENTJODS.2021.89577.1478.
Squamous cell carcinoma (SCC) comprises over 90% of oral malignancies. Cisplatin, as a selective chemotherapy agent to treat SCC, has many side effects despite its high effectiveness. There are some studies on the effects of bromelain derived from pineapple stems on different malignancies.
The aim of this study was to investigate the effect of bromelain alone and in combination with Cisplatin on oral squamous cell carcinoma (OSCC) and fibroblast cell lines.
In this interventional study, the HN5 cell line of OSCC and fibroblast cell line were treated with different concentrations of bromelain alone and in combination with cisplatin. Cell viability test was performed after 24, 48 and 72 hours using MTT (3-)4,5-dimethylthiazol-2-yl(-2,5 diphenyl tetrazolium bromide) assay. In the final stage, the drug-treated cells underwent flow cytometry to assess apoptosis patterns. Data were analyzed using SPSS 17, ANOVA (for general comparison of groups) and LSD post hoc tests (for comparison two groups). p< 0.05 was considered statistically significant.
The findings suggested that although bromelain showed toxic effects on HN5 cancer cells, its combination with Cisplatin resulted in little improvement in its effectiveness. Bromelain alone and in combination with Cisplatin presented cytotoxic effects against fibroblasts, which depended on the dosage and time exposure (p< 0.05). The flow cytometry results did not support the superior effect of the combination of two medications over Cisplatin alone (p> 0.05).
According to the findings, although adding bromelain to Cisplatin reduced toxicity on normal tissues, the combination of these two drugs did not increase the anticancer effect of Cisplatin. Thus, bromelain in combination with Cisplatin is not recommended as an adjuvant drug for OSCC.
鳞状细胞癌（SCC）占口腔恶性肿瘤的90%以上。顺铂作为治疗SCC的选择性化疗药物，尽管疗效高，但有许多副作用。有一些关于菠萝茎中提取的菠萝蛋白酶对不同恶性肿瘤影响的研究。
本研究的目的是调查菠萝蛋白酶单独及与顺铂联合使用对口腔鳞状细胞癌（OSCC）和成纤维细胞系的影响。
在这项干预性研究中，OSCC的HN5细胞系和成纤维细胞系分别用不同浓度的菠萝蛋白酶单独及与顺铂联合处理。在24、48和72小时后使用MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四氮唑）法进行细胞活力测试。在最后阶段，对药物处理的细胞进行流式细胞术以评估凋亡模式。使用SPSS 17软件进行数据分析，采用方差分析（用于组间总体比较）和LSD事后检验（用于两组比较）。p<0.05被认为具有统计学意义。
研究结果表明，尽管菠萝蛋白酶对HN5癌细胞显示出毒性作用，但其与顺铂联合使用在有效性方面几乎没有改善。菠萝蛋白酶单独及与顺铂联合使用对成纤维细胞呈现细胞毒性作用，这取决于剂量和暴露时间（p<0.05）。流式细胞术结果不支持两种药物联合使用比单独使用顺铂具有更好的效果（p>0.05）。
根据研究结果，尽管在顺铂中添加菠萝蛋白酶可降低对正常组织的毒性，但这两种药物联合使用并未增加顺铂的抗癌效果。因此，不推荐菠萝蛋白酶与顺铂联合作为OSCC的辅助药物。
