Department of Agricultural Science, Graduate School of Sustainability Science, Tottori, Tottori 680-8553, Japan.
Department of Bioresources Science, The United Graduate School of Agriculture, Tottori University, Tottori, Japan.
Biosci Biotechnol Biochem. 2023 Jan 24;87(2):171-178. doi: 10.1093/bbb/zbac187.
The FMRFamide-like peptides (FLPs) are conserved in both free-living and parasitic nematodes. This molecular genetic study verified the relevance of the flp-1 gene, which is conserved in many nematode species, to the larval development of the free-living soil nematode Caenorhabditis elegans. Using C. elegans as a model, we found that: (1) FLP-1 suppressed larval development, resulting in diapause; (2) the secretion of FLP-1, which is produced in AVK head neurons, was suppressed by the presence of food (Escherichia coli) as an environmental factor to continue larval development; (3) the FLP-1 reduced the production and secretion of DAF-28, which is produced in ASI head neurons and is the predominant insulin-like peptide (INS) present. FLP-1 is conserved in many species of plant-parasitic root-knot nematodes that cause severe damage to crops. Therefore, our findings may provide insight into the development of new nematicides that can disturb their infection and development.
类脑啡肤样肽（FLPs）在自由生活和寄生线虫中都保守存在。这项分子遗传学研究证实了 flp-1 基因的相关性，该基因在许多线虫物种中都保守存在，与自由生活的土壤线虫秀丽隐杆线虫的幼虫发育有关。我们利用秀丽隐杆线虫作为模型，发现：（1）FLP-1 抑制幼虫发育，导致休眠；（2）作为环境因素的食物（大肠杆菌）存在时，AVK 头部神经元产生的 FLP-1 分泌受到抑制，以继续幼虫发育；（3）FLP-1 减少了 ASI 头部神经元产生的、主要的胰岛素样肽（INS）DAF-28 的产生和分泌。FLP-1 在引起作物严重损害的许多植物寄生根结线虫物种中都保守存在。因此，我们的发现可能为开发干扰其感染和发育的新型杀线虫剂提供思路。
