Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Methods Mol Biol. 2023;2592:75-88. doi: 10.1007/978-1-0716-2807-2_5.
We describe step-by-step methods to label human pancreatic islet cells and murine insulinoma cells and their subsequent transplantation into type I diabetic mouse models with a focus on in vivo imaging using clinically applicable scanners. We also cover islets that are microencapsulated within alginate hydrogels loaded with imaging agents. By following these methods, it is possible to image cell grafts using T-weighted and T/T*-weighted H magnetic resonance imaging (MRI), F MRI, computed tomography, ultrasound imaging, and bioluminescence imaging in vivo. Considering a myriad of factors that may affect the outcome of proper in vivo detection, we discuss potential issues that may be encountered during and after the process of labeling. The ultimate goal is to use these in vivo imaging approaches to determine and optimize naked and encapsulated islet cell survival, therapeutic function, and engraftment procedures.
我们描述了逐步标记人胰岛细胞和鼠胰岛素瘤细胞的方法，以及将其随后移植到 I 型糖尿病小鼠模型中的方法，重点是使用临床适用的扫描仪进行体内成像。我们还涵盖了微囊化在载有成像剂的藻酸盐水凝胶内的胰岛。通过遵循这些方法，可以使用 T 加权和 T/T*-加权 H 磁共振成像（MRI）、F MRI、计算机断层扫描、超声成像和体内生物发光成像来对细胞移植物进行成像。考虑到可能影响体内检测效果的诸多因素，我们讨论了在标记过程中和之后可能遇到的潜在问题。最终目标是使用这些体内成像方法来确定和优化裸细胞和包封胰岛细胞的存活、治疗功能和移植程序。
