2 型糖尿病中的 microRNAs：重点关注胰岛中的 microRNA 谱分析。

MicroRNAs in Type 2 Diabetes: Focus on MicroRNA Profiling in Islets of Langerhans.

机构信息

Unit of Islet Cell Exocytosis, Department of Clinical Sciences - Malmö, Lund University Diabetes Centre (LUDC), Lund University, Clinical Research Centre, and Skåne University Hospital (SUS), Malmö, Sweden.

出版信息

Methods Mol Biol. 2023;2592:113-142. doi: 10.1007/978-1-0716-2807-2_8.

DOI:10.1007/978-1-0716-2807-2_8

PMID:36507989

Abstract

Differential expression of microRNAs (miRNAs) is observed in many diseases including type 2 diabetes (T2D). Insulin secretion from pancreatic beta cells is central for the regulation of blood glucose levels and failure to release enough insulin results in hyperglycemia and T2D. The importance in T2D pathogenesis of single miRNAs in beta cells has been described; however, to get the full picture, high-throughput miRNA sequencing is necessary. Here we describe a method using small RNA sequencing, from sample preparation to expression analysis using bioinformatic tools. In the end, a tutorial on differential expression analysis is presented in R using publicly available data.

摘要

在许多疾病中都观察到微小 RNA（miRNAs）的差异表达，包括 2 型糖尿病（T2D）。胰腺β细胞的胰岛素分泌对于调节血糖水平至关重要，而不能释放足够的胰岛素会导致高血糖和 T2D。已经描述了单个 miRNAs 在β细胞中在 T2D 发病机制中的重要性；然而，为了全面了解情况，需要进行高通量 miRNA 测序。在这里，我们描述了一种使用小 RNA 测序的方法，从样品制备到使用生物信息学工具进行表达分析。最后，我们使用公共可用数据在 R 中呈现了差异表达分析的教程。