Huang I-Shen, Chen Wei-Jen, Wang Zhong-Lin, Li Li-Hua, Chen Yu-Kuang, Wu Yuh-Lin, Brannigan Robert E, Juan Chi-Chang, Huang William J
Department of Urology, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
Department of Physiology, School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan, ROC.
J Chin Med Assoc. 2023 Feb 1;86(2):197-206. doi: 10.1097/JCMA.0000000000000838. Epub 2022 Nov 4.
In rodent models, low-intensity extracorporeal shock wave therapy has been shown to negatively impact semen concentration after treatment on the penis, implying that the reproductive system in close proximity may be indirectly affected by this modality. We hypothesized that shock waves are detrimental to spermatogenesis, and the aim of this study was to evaluate the effect of shock waves on spermatogenesis after direct shockwave treatment on testes using different energy settings.
Twenty-five male Sprague Dawley rats, 8 weeks old, were divided into five groups, including one control group and four treatment groups each treated using shock waves of different intensities. All rats in the treatment groups received 2000 shocks on the left testis twice a week for 4 weeks, with shock wave intensity and frequency varied by treatment group: 0.1 mJ/mm 2 at 4 Hz for Group A, 0.15 mJ/mm 2 at 4 Hz for Group B, 0.35 mJ/mm 2 at 4 Hz for Group C, and 0.55mJ/mm 2 at 3 Hz for Group D. At the end of the experiment, sperm collected from the epididymis was evaluated for concentration and motility. Testicular spermatogenesis, the apoptotic index of germ cells, and the expression of a meiotic-specific gene were also analyzed.
The treatment group receiving shock wave intensity at 0.55 mJ/mm 2 showed a significant decrease in sperm concentration, motility, and Johnsen score as compared to other groups. The apoptotic index of spermatogenic cells increased as the intensity of the shock wave treatment escalated, and reach a statistically significant difference at 4 weeks posttreatment. Treating testes with intensity levels of 0.55 mJ/mm 2 at 3 Hz interfere with the quality or quantity of spermatogenesis and also increases in spermatogenic cell apoptosis, whereas the expression of the SYCP3 gene significantly decreased after treatment with intensity levels of 0.10 mJ/mm 2 , 0.15 mJ/mm 2 , and 0.35 mJ/mm 2 at 4 Hz.
Treating testes with intensity levels of 0.55 mJ/mm 2 at 3 Hz interfere with the quality or quantity of spermatogenesis and also increases spermatogenic cell apoptosis, whereas the expression of the SYCP3 gene significantly decreased after treatment with intensity levels of 0.10 mJ/mm 2 , 0.15 mJ/mm 2 , and 0.35 mJ/mm 2 at 4 Hz.
在啮齿动物模型中，低强度体外冲击波疗法已被证明在阴茎治疗后会对精液浓度产生负面影响，这意味着附近的生殖系统可能会受到这种治疗方式的间接影响。我们假设冲击波对精子发生有害，本研究的目的是评估在使用不同能量设置对睾丸进行直接冲击波治疗后，冲击波对精子发生的影响。
将25只8周龄的雄性Sprague Dawley大鼠分为五组，包括一个对照组和四个治疗组，每个治疗组使用不同强度的冲击波进行治疗。治疗组的所有大鼠每周两次在左侧睾丸接受2000次冲击波，持续4周，冲击波强度和频率因治疗组而异：A组为4Hz、0.1mJ/mm²；B组为4Hz、0.15mJ/mm²；C组为4Hz、0.35mJ/mm²；D组为3Hz、0.55mJ/mm²。实验结束时，对从附睾收集的精子进行浓度和活力评估。还分析了睾丸精子发生、生殖细胞凋亡指数和减数分裂特异性基因的表达。
与其他组相比，接受0.55mJ/mm²冲击波强度治疗的组精子浓度、活力和约翰森评分显著降低。随着冲击波治疗强度的增加，生精细胞的凋亡指数升高，在治疗后4周达到统计学显著差异。以3Hz、0.55mJ/mm²强度水平治疗睾丸会干扰精子发生的质量或数量，并增加生精细胞凋亡，而在以4Hz、0.10mJ/mm²、0.15mJ/mm²和0.35mJ/mm²强度水平治疗后，SYCP3基因的表达显著降低。
以3Hz、0.55mJ/mm²强度水平治疗睾丸会干扰精子发生的质量或数量，并增加生精细胞凋亡，而在以4Hz、0.10mJ/mm²、0.15mJ/mm²和0.35mJ/mm²强度水平治疗后，SYCP3基因的表达显著降低。
