Wang Shuang-Shuang, Dou Quanli, Sui Changling, Yuan Guangyan, Zeng Boping
College of Biology and Agricultural Science, Zunyi Normal University, Zunyi, 563006, China.
College of Biology and Agricultural Science, Zunyi Normal University, Zunyi, 563006, China.
Gene Expr Patterns. 2023 Mar;47:119298. doi: 10.1016/j.gep.2022.119298. Epub 2022 Dec 10.
Squalene epoxidase catalyzes the oxidation of squalene to 2,3-oxo-squalene (BsSE1), and is the key rate limiting enzyme in the synthesis of triterpenoids and sterols in plants. This study focused on the basic aspects of BsSE1 including the sequence information, sub-cellular localization expression patterns of BsSE1. Using to the sequence information of Bletilla striata transcriptome, the full-length CDS of BsSE1 gene was amplified. The physicochemical properties and structural characteristics of BsSE1 protein were analyzed by bioinformatics analysis software, and vector was constructed to analyze the protein locations and expression patterns. The results showed that the CDS of BsSE1 gene was 1542 bp, encoding 513 amino acids. BsSE1 protein is a hydrophobic protein with two transmembrane domains but no signal peptides. It is localied in the endoplasmic reticulum membrane and belongs to the typical squalene epoxidase gene. BsSE1 has the closest genetic relationship with SE protein of Dendrobium officinale and Phalaenopsis equestris. The expression level of BsSE1 was higher in pseudobulblet of Bletilla striata seedlings, followed by roots, and lower in seedling stems. After SA induction, the expression of BsSE1 in Bletilla striata showed significant changes, increased first, then decreased, finally increase again. The results provide a basis for further study of this gene family in plants.
角鲨烯环氧酶催化角鲨烯氧化生成2,3-氧化角鲨烯（BsSE1），是植物三萜类化合物和甾醇合成中的关键限速酶。本研究聚焦于BsSE1的基础方面，包括其序列信息、亚细胞定位及表达模式。利用白及转录组的序列信息，扩增得到BsSE1基因的全长CDS。通过生物信息学分析软件分析BsSE1蛋白的理化性质和结构特征，并构建载体分析蛋白定位和表达模式。结果表明，BsSE1基因的CDS为1542 bp，编码513个氨基酸。BsSE1蛋白是一种疏水蛋白，有两个跨膜结构域但无信号肽。它定位于内质网膜，属于典型的角鲨烯环氧酶基因。BsSE1与铁皮石斛和蝴蝶兰的SE蛋白亲缘关系最近。BsSE1在白及幼苗假鳞茎中的表达水平较高，其次是根，在幼苗茎中较低。SA诱导后，白及中BsSE1的表达出现显著变化，先升高，再降低，最后又升高。这些结果为进一步研究该植物基因家族提供了依据。
