Gupta Bipin, Yang George, Petrauskene Olga, Key Marc
Diagnostic BioSystems, Pleasanton, CA, USA.
Key Biomedical Services, Ojai, CA, USA.
Methods Mol Biol. 2023;2593:35-50. doi: 10.1007/978-1-0716-2811-9_3.
Various staining strategies and color combinations have been developed to perform single and double immunohistochemical staining on biological samples. However, until recently, the lack of appropriate chromogen color combinations has severely limited many of these methods. Fortunately, this situation has dramatically improved with the introduction of new chromogens and methods of analysis. This article reviews recent trends in multicolor immunohistochemical staining methods that are finding broad applications in both research and clinical laboratories.
已经开发出各种染色策略和颜色组合,用于对生物样品进行单重和双重免疫组织化学染色。然而,直到最近,缺乏合适的显色剂颜色组合严重限制了其中许多方法。幸运的是,随着新显色剂和分析方法的引入,这种情况有了显著改善。本文综述了多色免疫组织化学染色方法的最新趋势,这些方法在研究和临床实验室中都有广泛应用。