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OptiView和EnVision FLEX检测系统在常规细胞病理学实践中十种最常用诊断标志物免疫细胞化学染色方案中的验证与应用

Validation and Implementation of OptiView and EnVision FLEX Detection Systems for Immunocytochemical Staining Protocols of the Ten Most Commonly Used Diagnostic Markers in Routine Cytopathological Practice.

作者信息

Dremelj Anja, Miceska Simona, Kuhar Anamarija, Nolde Natasa, Kloboves-Prevodnik Veronika

机构信息

Institute of Oncology Ljubljana, Zaloska cesta 2, 1000 Ljubljana, Slovenia.

Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000 Ljubljana, Slovenia.

出版信息

Diagnostics (Basel). 2024 Mar 21;14(6):657. doi: 10.3390/diagnostics14060657.

DOI:10.3390/diagnostics14060657
PMID:38535077
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10968775/
Abstract

The withdrawal of the iView detection system (iV) forced many cytopathology laboratories, including ours, to substitute immunocytochemical (ICC) staining protocols for routine practice with other detection systems. Our objective was to optimize, validate, and implement ICC protocols using OptiView (OV) and EnVision FLEX (EnV) detection systems, comparing the results with those obtained using iV. Residual cytologic samples with known diagnoses were used, testing antibodies for the ten most common markers in routine cytopathology diagnostics (calretinin, Ber-EP4, MOC-31, CKAE1/AE3, CK5/6, CD68, LCA, desmin, HBME-1, and WT1). Different staining parameters were tested using OV on BenchMark ULTRA and EnV on Dako Omnis immunostainer, respectively. Optimal staining protocols were then selected and validated on 10 positive and 10 negative cases. The staining results were compared with iV protocols through evaluation of UK NEQAS and internal scores. The optimal staining protocols with OV and EnV demonstrated similar or superior results compared to the existing iV protocols, with slightly stronger intensity regarding positive cells. We have successfully established and validated optimal ICC staining protocols for commonly used markers in routine cytopathology practice. These protocols may benefit other laboratories using similar staining platforms. However, the challenge regarding standardizing ICC protocols across different cytopathology laboratories remains unresolved.

摘要

iView检测系统（iV）的停用迫使包括我们实验室在内的许多细胞病理学实验室，在常规操作中用免疫细胞化学（ICC）染色方案替代其他检测系统。我们的目标是优化、验证并实施使用OptiView（OV）和EnVision FLEX（EnV）检测系统的ICC方案，并将结果与使用iV获得的结果进行比较。使用已知诊断结果的剩余细胞学样本，检测常规细胞病理学诊断中十种最常见标志物的抗体（钙视网膜蛋白、Ber-EP4、MOC-31、细胞角蛋白AE1/AE3、细胞角蛋白5/6、CD68、白细胞共同抗原、结蛋白、HBME-1和WT1）。分别在BenchMark ULTRA上使用OV和在Dako Omnis免疫染色仪上使用EnV测试不同的染色参数。然后选择最佳染色方案，并在10例阳性和10例阴性病例上进行验证。通过评估英国国家外部质量评估计划（UK NEQAS）和内部评分，将染色结果与iV方案进行比较。与现有的iV方案相比，使用OV和EnV的最佳染色方案显示出相似或更好的结果，阳性细胞的强度略强。我们已成功建立并验证了常规细胞病理学实践中常用标志物的最佳ICC染色方案。这些方案可能会使使用类似染色平台的其他实验室受益。然而，不同细胞病理学实验室之间ICC方案标准化的挑战仍未解决。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/10968775/b228b1574467/diagnostics-14-00657-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/10968775/b0f5f6605287/diagnostics-14-00657-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/10968775/c53ab2ab6f21/diagnostics-14-00657-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/10968775/858ba9685ec5/diagnostics-14-00657-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/10968775/80fd57283f2a/diagnostics-14-00657-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/10968775/b228b1574467/diagnostics-14-00657-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/10968775/b0f5f6605287/diagnostics-14-00657-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/10968775/c53ab2ab6f21/diagnostics-14-00657-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/10968775/858ba9685ec5/diagnostics-14-00657-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/10968775/80fd57283f2a/diagnostics-14-00657-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/10968775/b228b1574467/diagnostics-14-00657-g005.jpg

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Cytopathological assessment is an accurate method for identifying immunophenotypic features and BRCA1/2 mutations of high-grade serous carcinoma from ascites.
细胞病理学评估是一种准确的方法，可用于识别腹水高等级浆液性癌的免疫表型特征和 BRCA1/2 突变。
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