Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, Urbana, IL 61802, USA.
Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.
Cell Rep. 2022 Dec 13;41(11):111802. doi: 10.1016/j.celrep.2022.111802.
Asymmetric localization of mRNAs is crucial for cell polarity and cell fate determination. By performing fractionation RNA-seq, we report here that a large number of maternal RNAs are associated with the ER in Xenopus oocytes but are released into the cytosol after oocyte maturation. We provide evidence that the majority of ER-associated RNA-binding proteins (RBPs) remain associated with the ER after oocyte maturation. However, all ER-associated RBPs analyzed exhibit reduced binding to some of their target RNAs after oocyte maturation. Our results further show that the ER is remodeled massively during oocyte maturation, leading to the formation of a widespread tubular ER network in the animal hemisphere that is required for the asymmetric localization of mRNAs in mature eggs. Thus, our findings demonstrate that dynamic regulation of RNA-ER association and remodeling of the ER are important for the asymmetric localization of RNAs during development.
mRNA 的不对称定位对于细胞极性和细胞命运决定至关重要。通过进行分级 RNA-seq，我们在这里报告，大量的母源 RNA 与 Xenopus 卵母细胞中的 ER 相关，但在卵母细胞成熟后被释放到细胞质中。我们提供的证据表明，大多数与 ER 相关的 RNA 结合蛋白（RBPs）在卵母细胞成熟后仍与 ER 相关联。然而，在卵母细胞成熟后，所有分析的与 ER 相关的 RBPs 对其一些靶 RNA 的结合能力均降低。我们的结果还表明，在卵母细胞成熟过程中，ER 会发生大规模重塑，导致在动物半球中形成广泛的管状 ER 网络，这对于成熟卵子中 mRNA 的不对称定位是必需的。因此，我们的研究结果表明，RNA-ER 结合的动态调节和 ER 的重塑对于发育过程中 RNA 的不对称定位很重要。
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