Gon G, Nakamura F, Ishikawa H
Department of Anatomy, Jikei University School of Medicine, Tokyo, Japan.
Cell Tissue Res. 1987 Oct;250(1):29-33. doi: 10.1007/BF00214650.
Hemipituitary glands from 30 rats were isotransplanted under renal capsules. At 1, 2, 4, 7 and 20 days after transplantation, the grafts were examined by light and electron microscopy. Two days after transplantation, the central area of graft showed necrosis; however, the peripheral area, where marginal cells of Rathke's cleft were ingesting the remnants of necrotic glandular cells, survived. Four days after transplantation, mitotic figures of marginal cells were observed. Seven days after transplantation, the damaged area of the graft disappeared, and Rathke's cleft was completely lined by marginal cells. Remnants of necrotic glandular cells were not seen in intercellular spaces or in the cytoplasm of marginal cells. Cystlike structures formed in the grafts; some were connected to Rathke's cleft by narrow cavities. The cavity-lining cells of the cysts were agranular and similar to those that lined Rathke's cleft. At 20 days after transplantation, granular cavity-lining cells appeared. It is suggested that marginal cells of immature rats can differentiate into granular cells.
将30只大鼠的半垂体移植到肾被膜下。在移植后1天、2天、4天、7天和20天，通过光镜和电镜对移植物进行检查。移植后2天，移植物的中央区域出现坏死；然而，周边区域存活下来，在该区域拉特克裂的边缘细胞正在吞噬坏死腺细胞的残余物。移植后4天，观察到边缘细胞的有丝分裂象。移植后7天，移植物的受损区域消失，拉特克裂完全由边缘细胞衬里。在细胞间隙或边缘细胞的细胞质中未见坏死腺细胞的残余物。移植物中形成了囊样结构；一些通过狭窄的腔与拉特克裂相连。囊肿的腔衬细胞无颗粒，与拉特克裂的衬里细胞相似。移植后20天，出现了有颗粒的腔衬细胞。提示未成熟大鼠的边缘细胞可分化为颗粒细胞。
