Yalmanci Dilara, Dertli Enes, Tekin-Cakmak Zeynep Hazal, Karasu Salih
Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul, Turkey.
Department of Nutrition and Dietetics, Health Sciences Faculty, Istinye University, İstanbul 34010, Turkey.
Int J Biol Macromol. 2023 Jan 31;226:772-779. doi: 10.1016/j.ijbiomac.2022.12.069. Epub 2022 Dec 13.
This study aimed to investigate the potential usage of exopolysaccharide (EPS) produced by Leuconostoc lactis GW-6 species as an emulsifier in a low-fat mayonnaise by the formation of a complex with whey protein isolate (WPI) to improve rheological properties, emulsion, and oxidative stability. For the determination of rheological properties, the flow behavior, frequency sweep, and 3-ITT rheological properties of low-fat mayonnaise samples were studied. All samples showed shear thinning, viscoelastic solid-like, and recoverable character. The K and n values for the mayonnaise samples were determined as 24.529-174.403 Pa.s and 0.166-0.304, respectively, indicating that shear-thinning characters could be improved with WPI-EPS interaction. The higher K' and K″ values of all low-fat samples prepared with EPS-WPI than the low-fat control sample explained the synergistic effect of EPS and WPI. Importantly, no effect was observed when WPI was used as alone as an emulsifier. Oxidative stability was tested by OXITEST and IP values of samples prepared by WPI and EPS were compared to control samples. In conclusion, the results of this study showed that the EPS and WPI interaction can significantly affect the rheological properties and emulsion and oxidative stability of mayonnaise samples.
本研究旨在探讨乳酸明串珠菌GW-6菌株产生的胞外多糖(EPS)与乳清分离蛋白(WPI)形成复合物后,作为乳化剂在低脂蛋黄酱中的潜在用途,以改善其流变学特性、乳化性能和氧化稳定性。为测定流变学特性,研究了低脂蛋黄酱样品的流动行为、频率扫描和3-ITT流变学特性。所有样品均表现出剪切变稀、粘弹性固体状和可恢复的特性。蛋黄酱样品的K值和n值分别测定为24.529-174.403 Pa·s和0.166-0.304,表明WPI-EPS相互作用可改善剪切变稀特性。与低脂对照样品相比,用EPS-WPI制备的所有低脂样品的K'值和K''值更高,这解释了EPS和WPI的协同作用。重要的是,单独使用WPI作为乳化剂时未观察到效果。通过OXITEST测试氧化稳定性,并将由WPI和EPS制备的样品的IP值与对照样品进行比较。总之,本研究结果表明,EPS和WPI的相互作用可显著影响蛋黄酱样品的流变学特性、乳化性能和氧化稳定性。