Richter Anne, Pietsch Michael, Harmsen Dag, Juraschek Katharina, Lang Christina, Mellmann Alexander, Middendorf-Bauchart Barbara, Pulz Matthias, Roth Sarah, Schuh Elisabeth, Fruth Angelika, Flieger Antje
Fachgebiet 11: Bakterielle darmpathogene Erreger und Legionellen, Robert Koch-Institut (RKI), Burgstr. 37, 38855, Wernigerode, Deutschland.
Poliklinik für Parodontologie und Zahnerhaltung, Universitätsklinikum Münster, Münster, Deutschland.
Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. 2023 Jan;66(1):84-91. doi: 10.1007/s00103-022-03628-6. Epub 2022 Dec 16.
In order to improve patient care and to increase food safety within the framework of One Health, the project "Integrated Genomic Surveillance of Zoonotic Agents (IGS-Zoo)" aims to develop concepts for a genomic surveillance of Shiga toxin(Stx)-producing and enterohemorrhagic Escherichia coli (STEC/EHEC) in Germany.
An online survey was conducted to assess the currently available and applied STEC/EHEC typing methods in the federal laboratories of veterinary regulation, food control, and public health service.
Twenty-six questionnaires from 33 participants were evaluated with regard to STEC/EHEC. The number of STEC/EHEC-suspected samples that the laboratories process per year ranges between 10 and 3500, and out of these they obtain between 3 and 1000 pathogenic isolates. Currently the most frequently used typing method is the determination of Stx- and intimin-coding genes using polymerase chain reaction (PCR). Whole genome sequencing (WGS) is currently used by eight federal state laboratories, and nine are planning to implement it in the future. The most common obstacle for further typing of STEC/EHEC is that isolation from sample material is often unsuccessful despite apparent PCR detection of the stx genes.
The results of the survey should facilitate the integration of the analysis methods developed in the project and emphasize the target groups' individual needs for corresponding training concepts.
为了在“同一健康”框架下改善患者护理并提高食品安全，“人畜共患病原体综合基因组监测（IGS-Zoo）”项目旨在开发德国产志贺毒素（Stx）和肠出血性大肠杆菌（STEC/EHEC）基因组监测的概念。
开展了一项在线调查，以评估兽医监管、食品控制和公共卫生服务联邦实验室中目前可用和应用的STEC/EHEC分型方法。
对33名参与者的26份问卷进行了STEC/EHEC方面的评估。各实验室每年处理的疑似STEC/EHEC样本数量在10至3500之间，其中能获得3至1000株致病性分离株。目前最常用的分型方法是使用聚合酶链反应（PCR）测定Stx和intimin编码基因。目前有八个联邦州实验室使用全基因组测序（WGS），九个计划在未来采用。STEC/EHEC进一步分型的最常见障碍是，尽管stx基因在PCR检测中呈阳性，但从样本材料中分离往往不成功。
调查结果应有助于整合项目中开发的分析方法，并强调目标群体对相应培训概念的个性化需求。
