Calbiague García Víctor, Chen Yiyi, Cádiz Bárbara, Wang Lan, Paquet-Durand François, Schmachtenberg Oliver
PhD Program in Neuroscience, Universidad de Valparaíso, Valparaíso, Chile; CINV, Instituto de Biología, Universidad de Valparaíso, Chile.
Institute for Ophthalmic Research, University of Tübingen, Germany.
Exp Eye Res. 2023 Jan;226:109352. doi: 10.1016/j.exer.2022.109352. Epub 2022 Dec 14.
Müller cells, the glial cells of the retina, provide metabolic support for photoreceptors and inner retinal neurons, and have been proposed as source of the significant lactate production of this tissue. To better understand the role of lactate in retinal metabolism, we expressed a lactate and a glucose nanosensor in organotypic mouse retinal explants cultured for 14 days, and used FRET imaging in acute vibratome sections of the explants to study metabolite flux in real time. Pharmacological manipulation with specific monocarboxylate transporter (MCT) inhibitors and immunohistochemistry revealed the functional expression of MCT1, MCT2 and MCT4 in Müller cells of retinal explants. The introduction of FRET nanosensors to measure key metabolites at the cellular level may contribute to a better understanding of heretofore poorly understood issues in retinal metabolism.
缪勒细胞是视网膜的神经胶质细胞，为光感受器和视网膜内层神经元提供代谢支持，并且被认为是该组织中大量乳酸产生的来源。为了更好地理解乳酸在视网膜代谢中的作用，我们在培养14天的小鼠视网膜器官型外植体中表达了乳酸和葡萄糖纳米传感器，并利用外植体急性振动切片中的荧光共振能量转移成像实时研究代谢物通量。用特定的单羧酸转运体（MCT）抑制剂进行药理操作和免疫组织化学揭示了视网膜外植体缪勒细胞中MCT1、MCT2和MCT4的功能性表达。引入FRET纳米传感器在细胞水平测量关键代谢物可能有助于更好地理解视网膜代谢中迄今了解甚少的问题。
