Chang Jinyuan, Sun Yang, Meng Xianxi, Zeng Fanglin, Wang Xiancheng
Department of Burn and Plastic Surgery, The Second Xiangya Hospital of Central South University, No. 139, Shaoshan South Road, Furong District, Changsha, Hunan, 410011, China.
Regen Med. 2023 Feb;18(2):137-153. doi: 10.2217/rme-2022-0123. Epub 2022 Dec 19.
This study aimed to explore the role of EGFL7 in the healing process of refractory diabetic wounds. Epidermal stem cells (ESCs) were isolated from healthy mice and diabetic mice, identified by immunofluorescence, transfected with EGFL7 overexpression and silencing lentiviral vectors, and treated with Notch pathway inhibitor (DAPT). SiEGFL7 significantly inhibited the proliferation, invasion and migration of ESCs of healthy mice. DAPT prominently inhibited the expressions of Notch1, Notch2, Hes1 and Jag1 in ESCs of healthy mice induced by overexpressed EGFL7. Overexpressed EGFL7 promoted wound healing in diabetic mice with refractory wounds. EGFL7 affects the proliferation and migration of ESCs in refractory diabetic wounds by regulating the Notch signaling pathway.
本研究旨在探讨表皮生长因子样蛋白7（EGFL7）在难治性糖尿病伤口愈合过程中的作用。从健康小鼠和糖尿病小鼠中分离表皮干细胞（ESCs），通过免疫荧光进行鉴定，用EGFL7过表达和沉默慢病毒载体转染，并使用Notch信号通路抑制剂（DAPT）进行处理。SiEGFL7显著抑制健康小鼠ESCs的增殖、侵袭和迁移。DAPT显著抑制由过表达EGFL7诱导的健康小鼠ESCs中Notch1、Notch2、Hes1和Jag1的表达。过表达EGFL7促进难治性伤口糖尿病小鼠的伤口愈合。EGFL7通过调节Notch信号通路影响难治性糖尿病伤口中ESCs的增殖和迁移。
