在伊朗北部戈尔甘的转诊医院的空气、表面和废水中检测到严重急性呼吸综合征冠状病毒2（SARS-CoV-2）基因组。
Detection of SARS-CoV-2 genome in the air, surfaces, and wastewater of the referral hospitals, Gorgan, north of Iran.
作者信息
Ramezani Ziarani Farzad, Tahamtan Alireza, Safari Hasan, Tabarraei Alijan, Dadban Shahamat Yousef
机构信息
Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
Department of Environmental Health Engineering, Faculty of Health, Environmental Health Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
出版信息
Iran J Microbiol. 2022 Oct;14(5):617-623. doi: 10.18502/ijm.v14i5.10954.
BACKGROUND AND OBJECTIVES
Coronavirus disease 2019 (COVID-19) is a pandemic caused by the novel virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Knowing the virus's behavior and its persistence in different environments are crucial and will lead to the proper management of the disease. In this study, air, surface, and sewage samples were taken from different parts of referral hospitals for COVID-19.
MATERIALS AND METHODS
Air samples were taken with impinger, surface samples with swabs, and sewage samples were taken from the hospital wastewater treatment plant. After viral genome extraction, a real-time RT-PCR test was applied to confirm the presence of SARS-CoV-2 RNA in the collected samples.
RESULTS
The virus genome could be traced in the wards and wastewater related to hospitalized COVID-19 patients. Overally, 29%, 16%, and 37.5% of air, surface, and sewage samples were positive for the SARS-CoV-2 genome, respectively.
CONCLUSION
Findings of such studies provide valuable results regarding the degree of contamination of hospital environments and the risk of virus transmission in different environments and among hospital staff and patients.
背景与目的
2019冠状病毒病（COVID-19）是由新型病毒严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起的大流行病。了解该病毒的行为及其在不同环境中的持久性至关重要，这将有助于对该疾病进行妥善管理。在本研究中，从收治COVID-19患者的转诊医院的不同区域采集了空气、表面和污水样本。
材料与方法
空气样本用冲击式采样器采集，表面样本用拭子采集，污水样本从医院污水处理厂采集。在提取病毒基因组后，应用实时逆转录聚合酶链反应（RT-PCR）检测来确认所采集样本中是否存在SARS-CoV-2 RNA。
结果
在与住院COVID-19患者相关的病房和废水中可检测到病毒基因组。总体而言，空气、表面和污水样本中SARS-CoV-2基因组阳性率分别为29%、16%和37.5%。
结论
此类研究结果为医院环境的污染程度以及病毒在不同环境中、医院工作人员和患者之间传播的风险提供了有价值的结果。
Zotero 插件