Hensel W
Botanical Institute, University of Bonn, Federal Republic of Germany.
Exp Cell Res. 1987 Oct;172(2):377-84. doi: 10.1016/0014-4827(87)90395-8.
By application of cytochalasin D (10 micrograms/ml) the distribution and turnover of endoplasmic reticulum (ER) in root statocytes of cress (Lepidium sativum L.) was studied. After 7 min of incubation, the distal ER complex, in 20-h-old control statocytes consisting of stacked cisternae, was disintegrated and redistributed. The amyloplasts sedimented into the most distal part of the cell. When the incubation time was increased up to 4 h, ER was formed near the nucleus and accumulated at the proximal cell pole. Thus microfilaments are suggested to be involved in (i) stabilization of the distal ER complex and (ii) the ER translocation from the forming site into the distal cell pole. By morphometric measurements the volume of story II (story III) statocytes was calculated to be 2400 microns3 (3000 microns3), containing an ER area of 1824 microns2 (2400 microns2). From the net ER formation rate of 5.2 microns2/min (story II) and 4.6 microns2/min (story III) and the net decrease rate of 23 microns2/min (story II) and 39.2 microns2/min (story III), a total ER formation rate of about 27 microns2/min (story II) and 43 microns2/min (story III) was estimated.
通过应用细胞松弛素D（10微克/毫升），研究了水芹（独行菜）根平衡细胞中内质网（ER）的分布和周转情况。孵育7分钟后，在由堆叠的扁平囊组成的20小时龄对照平衡细胞中的远端内质网复合体解体并重新分布。淀粉体沉降到细胞的最远端部分。当孵育时间增加到4小时时，内质网在细胞核附近形成并在近端细胞极积累。因此，微丝被认为参与了（i）远端内质网复合体的稳定以及（ii）内质网从形成部位向远端细胞极的转运。通过形态测量，计算得出II层（III层）平衡细胞的体积为2400立方微米（3000立方微米），内质网面积为1824平方微米（2400平方微米）。根据II层（III层）内质网的净形成速率分别为5.2平方微米/分钟和4.6平方微米/分钟，以及净减少速率分别为23平方微米/分钟和39.2平方微米/分钟，估计II层（III层）内质网的总形成速率约为27平方微米/分钟和43平方微米/分钟。
