Hempel J, Höög J O, Jörnvall H
Department of Biochemistry, University of Pittsburgh, PA 15261.
FEBS Lett. 1987 Sep 28;222(1):95-8. doi: 10.1016/0014-5793(87)80198-9.
Comparison of existing protein and cDNA data for human liver mitochondrial aldehyde dehydrogenase reveals deviations in two segments. They are shown to correspond exactly to localized frameshifts in the cDNA data, which are likely to have three reading errors. After correction of the cDNA frameshifts, a deduced amino acid sequence corresponds exactly to the data established at the protein level. In addition, extension of the shifted frame into the cDNA corresponding to the mitochondrial leader sequence allows reinterpretation of that sequence. The new leader sequence is consistent with characteristics of such segments of other mitochondrial protein pro-forms. Furthermore, the sequence displays a homology, when centered around the cleavage site, with the leader sequence of rat liver carbamyl phosphate synthetase I, suggesting a novel similarity between mitochondrial targeting sequences of two different enzymes.
对人肝脏线粒体醛脱氢酶现有蛋白质和cDNA数据的比较显示，在两个片段中存在偏差。结果表明，它们与cDNA数据中定位的移码完全对应，可能存在三个读码错误。校正cDNA移码后，推导的氨基酸序列与蛋白质水平确定的数据完全一致。此外，将移位的读框延伸到对应线粒体前导序列的cDNA中，可以对该序列进行重新解释。新的前导序列与其他线粒体蛋白质前体形式的此类片段特征一致。此外，当以切割位点为中心时，该序列与大鼠肝脏氨甲酰磷酸合成酶I的前导序列具有同源性，这表明两种不同酶的线粒体靶向序列之间存在新的相似性。
